DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi’s sarcoma-associated herpesvirus latent replication

During latent infection, latency-associated nuclear antigen (LANA) of Kaposi’s sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protei...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2010-04, Vol.394 (4), p.934-939
Main Authors: Cha, Seho, Lim, Chunghun, Lee, Jae Young, Song, Yoon-Jae, Park, Junsoo, Choe, Joonho, Seo, Taegun
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ANTIGENS
Antigens, Nuclear - metabolism
Antigens, Viral - metabolism
Cell Line
DNA
DNA - metabolism
DNA-Binding Proteins - metabolism
DNA-PK
HERPES SIMPLEX
Herpesvirus
Herpesvirus 8, Human - physiology
Humans
Kaposi's sarcoma-associated herpesvirus
KSHV
Ku Autoantigen
LANA
Latent replication
Nuclear Proteins - metabolism
Phosphorylation
Protein Kinases - metabolism
PROTEINS
SARCOMAS
VIRAL DISEASES
Virus Latency
Virus Replication
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Summary:During latent infection, latency-associated nuclear antigen (LANA) of Kaposi’s sarcoma-associated herpesvirus (KSHV) plays important roles in episomal persistence and replication. Several host factors are associated with KSHV latent replication. Here, we show that the catalytic subunit of DNA protein kinase (DNA-PKcs), Ku70, and Ku86 bind the N-terminal region of LANA. LANA was phosphorylated by DNA-PK and overexpression of Ku70, but not Ku86, impaired transient replication. The efficiency of transient replication was significantly increased in the HCT116 (Ku86 +/−) cell line, compared to the HCT116 (Ku86 +/+) cell line, suggesting that the DNA-PK/Ku complex negatively regulates KSHV latent replication.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2010.03.086