Fucoxanthin exerts differing effects on 3T3-L1 cells according to differentiation stage and inhibits glucose uptake in mature adipocytes

• Published in: Biochemical and biophysical research communications 2011-06, Vol.409 (4), p.769-774
• Main Authors: Kang, Seong-Il, Ko, Hee-Chul, Shin, Hye-Sun, Kim, Hyo-Min, Hong, Youn-Suk, Lee, Nam-Ho, Kim, Se-Jae
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; 60 APPLIED LIFE SCIENCES; Adipocyte differentiation; Adipocytes - cytology; Adipocytes - drug effects; Adipocytes - metabolism; Adipogenesis - drug effects; Animals; CCAAT-Enhancer-Binding Protein-alpha - metabolism; CCAAT/enhancer-binding protein α; Fucoxanthin; GLUCOSE; Glucose - metabolism; Glucose uptake; INSULIN; MESSENGER-RNA; METABOLIC DISEASES; Mice; Peroxidase proliferator-activated receptor γ; PEROXIDASES; Petalonia; PHOSPHORYLATION; PPAR gamma - metabolism; RECEPTORS; Sterol Regulatory Element Binding Protein 1 - metabolism; STEROLS; SUBSTRATES; TRIGLYCERIDES; UPTAKE; Xanthophylls - pharmacology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2011.05.086

► Fucoxanthin enhances 3T3-L1 adipocyte differentiation at an early stage. ► Fucoxanthin inhibits 3T3-L1 adipocyte differentiation at intermediate and late stages. ► Fucoxanthin attenuates glucose uptake by inhibiting the phosphorylation of IRS in mature 3T3-L1 adipocytes. ► Fucoxanthin exerts its anti-obesity effect by inhibiting the differentiation of adipocytes at both intermediate and late stages, as well as glucose uptake in mature adipocytes. Progression of 3T3-L1 preadipocyte differentiation is divided into early (days 0–2, D0–D2), intermediate (days 2–4, D2–D4), and late stages (day 4 onwards, D4-). In this study, we investigated the effects of fucoxanthin, isolated from the edible brown seaweed Petalonia binghamiae, on adipogenesis during the three differentiation stages of 3T3-L1 preadipocytes. When fucoxanthin was applied during the early stage of differentiation (D0–D2), it promoted 3T3-L1 adipocyte differentiation, as evidenced by increased triglyceride accumulation. At the molecular level, fucoxanthin increased protein expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), sterol regulatory element-binding protein 1c (SREBP1c), and aP2, and adiponectin mRNA expression, in a dose-dependent manner. However, it reduced the expression of PPARγ, C/EBPα, and SREBP1c during the intermediate (D2–D4) and late stages (D4–D7) of differentiation. It also inhibited the uptake of glucose in mature 3T3-L1 adipocytes by reducing the phosphorylation of insulin receptor substrate 1 (IRS-1). These results suggest that fucoxanthin exerts differing effects on 3T3-L1 cells of different differentiation stages and inhibits glucose uptake in mature adipocytes.