Xanthorrhizol induced DNA fragmentation in HepG2 cells involving Bcl-2 family proteins

► We isolated xanthorrhizol, a sesquiterpenoid compound from Curcuma xanthorrhiza. ► Xanthorrhizol induced apoptosis in HepG2 cells as observed using SEM. ► Apoptosis in xanthorrhizol-treated HepG2 cells involved Bcl-2 family proteins. ► DNA fragmentation was observed in xanthorrhizol-treated HepG2...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2012-04, Vol.420 (4), p.834-838
Main Authors: Tee, Thiam-Tsui, Cheah, Yew-Hoong, Meenakshii, Nallappan, Mohd Sharom, Mohd Yusof, Azimahtol Hawariah, Lope Pihie
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Antineoplastic Agents - pharmacology
APOPTOSIS
Curcuma xanthorrhiza
DNA
DNA Fragmentation
DRUGS
Hep G2 Cells
HEPATOMAS
HepG2 human hepatoma cell
Humans
LIVER
Liver Neoplasms - metabolism
Phenols - pharmacology
PROTEINS
Proto-Oncogene Proteins c-bcl-2 - metabolism
SCANNING ELECTRON MICROSCOPY
Xanthorrhizol
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Summary:► We isolated xanthorrhizol, a sesquiterpenoid compound from Curcuma xanthorrhiza. ► Xanthorrhizol induced apoptosis in HepG2 cells as observed using SEM. ► Apoptosis in xanthorrhizol-treated HepG2 cells involved Bcl-2 family proteins. ► DNA fragmentation was observed in xanthorrhizol-treated HepG2 cells. ► DNA fragmentation maybe due to cleavage of PARP and DFF45/ICAD proteins. Xanthorrhizol is a plant-derived pharmacologically active sesquiterpenoid compound isolated from Curcuma xanthorrhiza. Previously, we have reported that xanthorrhizol inhibited the proliferation of HepG2 human hepatoma cells by inducing apoptotic cell death via caspase activation. Here, we attempt to further elucidate the mode of action of xanthorrhizol. Apoptosis in xanthorrhizol-treated HepG2 cells as observed by scanning electron microscopy was accompanied by truncation of BID; reduction of both anti-apoptotic Bcl-2 and Bcl-XL expression; cleavage of PARP and DFF45/ICAD proteins and DNA fragmentation. Taken together, these results suggest xanthorrhizol as a potent antiproliferative agent on HepG2 cells by inducing apoptosis via Bcl-2 family members. Hence we proposed that xanthorrhizol could be used as an anti-liver cancer drug for future studies.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2012.03.083