Synchronous intra-Golgi transport induces the release of Ca{sup 2+} from the Golgi apparatus

The mechanisms of secretory transport through the Golgi apparatus remain an issue of debate. The precise functional importance of calcium ions (Ca{sup 2+}) for intra-Golgi transport has also been poorly studied. Here, using different approaches to measure free Ca{sup 2+} concentrations in the cell c...

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Bibliographic Details
Published in:Experimental cell research 2010-08, Vol.316 (13)
Main Authors: Micaroni, Massimo, Perinetti, Giuseppe, Di Giandomenico, Daniele, Bianchi, Katiuscia, Spaar, Alexander, Mironov, Alexander A.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
CALCIUM IONS
CONCENTRATION RATIO
CYCLOHEXIMIDE
ENDOPLASMIC RETICULUM
ESTERS
FIBROBLASTS
FLUORESCENCE
GOLGI COMPLEXES
GTP-ASES
RECEPTORS
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Summary:The mechanisms of secretory transport through the Golgi apparatus remain an issue of debate. The precise functional importance of calcium ions (Ca{sup 2+}) for intra-Golgi transport has also been poorly studied. Here, using different approaches to measure free Ca{sup 2+} concentrations in the cell cytosol ([Ca{sup 2+}]{sub cyt}) and inside the lumen of the Golgi apparatus ([Ca{sup 2+}]{sub GA}), we have revealed transient increases in [Ca{sup 2+}]{sub cyt} during the late phase of intra-Golgi transport that are concomitant with a decline in the maximal [Ca{sup 2+}]{sub GA} restoration ability. Thus, this redistribution of Ca{sup 2+} from the Golgi apparatus into the cytosol during the movement of cargo through the Golgi apparatus appears to have a role in intra-Golgi transport, and mainly in the late Ca{sup 2+}-dependent phase of SNARE-regulated fusion of Golgi compartments.
ISSN:0014-4827
1090-2422
DOI:10.1016/J.YEXCR.2010.04.024