BDNF, produced by a TPO-stimulated megakaryocytic cell line, regulates autocrine proliferation

► It has been thought that BDNF is not produced in the megakaryocytic lineage. ► MEG-01 produces BDNF upon TPO stimulation and regulates its proliferation. ► BDNF accelerates proliferation of MEG-01 in an autocrine manner. ► BDNF may be an autocrine MEG-CSF, which regulates megakaryopoiesis. While h...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2012-10, Vol.427 (3), p.542-546
Main Authors: Tamura, Shogo, Nagasawa, Ayumi, Masuda, Yuya, Tsunematsu, Tetsuya, Hayasaka, Koji, Matsuno, Kazuhiko, Shimizu, Chikara, Ozaki, Yukio, Moriyama, Takanori
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Autocrine Communication - drug effects
Autocrine Communication - physiology
BDNF
BONE MARROW CELLS
BRAIN
Brain-Derived Neurotrophic Factor - biosynthesis
Cell Line
CELL PROLIFERATION
COLONY FORMING UNITS
Humans
IN VITRO
MEG-01
MEG-CSF
Megakaryocytes - cytology
Megakaryocytes - drug effects
Megakaryocytes - metabolism
Megakaryopoiesis
STEM CELLS
STIMULATION
Thrombopoietin - pharmacology
TPO
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► It has been thought that BDNF is not produced in the megakaryocytic lineage. ► MEG-01 produces BDNF upon TPO stimulation and regulates its proliferation. ► BDNF accelerates proliferation of MEG-01 in an autocrine manner. ► BDNF may be an autocrine MEG-CSF, which regulates megakaryopoiesis. While human platelets release endogenous brain-derived neurotrophic factor (BDNF) upon activation, a previous report on MEG-01, a megakaryocytic cell line, found no trace of BDNF production, and the pathophysiological function of platelet BDNF has remained elusive. In the present study, we demonstrate that MEG-01 produces BDNF in the presence of TPO and that this serves to potentiate cell proliferation. Our in vitro findings suggest that BDNF regulates MEG-01 proliferation in an autocrine manner, and we suggest that BDNF may be a physiological autocrine regulator of megakaryocyte progenitors.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2012.09.093