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Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype

Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in mono...

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Published in:Experimental cell research 2013-02, Vol.319 (3), p.144-152
Main Authors: de la Puente, Pilar, Ludeña, Dolores, López, Marta, Ramos, Jennifer, Iglesias, Javier
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creator de la Puente, Pilar
Ludeña, Dolores
López, Marta
Ramos, Jennifer
Iglesias, Javier
description Porcine mesenchymal stem cells (pMSCs) are an attractive source of cells for tissue engineering because their properties are similar to those of human stem cells. pMSCs can be found in different tissues but their dermal origin has not been studied in depth. Additionally, MSCs differentiation in monolayer cultures requires subcultured cells, and these cells are at risk of dedifferentiation when implanting them into living tissue. Following this, we attempted to characterize the MSCs phenotype of porcine dermal cells and to evaluate their cellular proliferation and differentiation in autologous fibrin scaffolds (AFSs). Dermal biopsies and blood samples were obtained from 12 pigs. Dermal cells were characterized by flow cytometry. Frozen autologous plasma was used to prepare AFSs. pMSC differentiation was studied in standard structures (monolayers and pellets) and in AFSs. The pMSCs expressed the CD90 and CD29 markers of the mesenchymal lineage. AFSs afforded adipogenic, osteogenic and chondrogenic differentiation. The porcine dermis can be proposed to be a good source of MSCs with adequate proliferative capacity and a suitable expression of markers. The pMSCs also showed optimal proliferation and differentiation in AFSs, such that these might serve as a promising autologous and implantable material for use in tissue engineering. ► Low fibrinogen concentration provides a suitable matrix for cell migration and differentiation. ► Autologous fibrin scaffolds is a promising technique in tissue engineering. ► Dermal cells are an easily accessible mesenchymal stem cell source. ► Fibrin scaffolds afforded adipogenic, osteogenic and chondrogenic differentiation.
doi_str_mv 10.1016/j.yexcr.2012.10.009
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identifier ISSN: 0014-4827
ispartof Experimental cell research, 2013-02, Vol.319 (3), p.144-152
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language eng
recordid cdi_osti_scitechconnect_22215482
source ScienceDirect Freedom Collection 2022-2024
subjects 60 APPLIED LIFE SCIENCES
Adipogenesis - drug effects
Animals
BIOPSY
BLOOD
Cell Culture Techniques
Cell Differentiation - drug effects
CELL PROLIFERATION
Cell Proliferation - drug effects
CONCENTRATION RATIO
Dermal cells
Dermis - cytology
Dermis - drug effects
Dermis - physiology
Differentiation
FIBRIN
Fibrin - chemistry
Fibrin - pharmacology
Fibrin scaffolds
FIBRINOGEN
Genotype & phenotype
Hogs
IMPLANTS
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - drug effects
Mesenchymal Stromal Cells - physiology
Osteogenesis - drug effects
Osteogenesis - physiology
PHENOTYPE
Porcine mesenchymal stem cells
Primary Cell Culture
STEM CELLS
SWINE
Tissue Engineering
Tissue Scaffolds - chemistry
Transplantation, Autologous
title Differentiation within autologous fibrin scaffolds of porcine dermal cells with the mesenchymal stem cell phenotype
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