Nuclear pool of phosphatidylinositol 4 phosphate 5 kinase 1α is modified by polySUMO-2 during apoptosis

•Nuclear pool of PIP5K is SUMOylated.•Enhancement of SUMOylated nuclear PIP5K during apoptosis.•Nuclear PIP5K is modified by polySUMO-1 during apoptosis.•Nuclear PIP5K is modified by polySUMO-2 chain during apoptosis. Phosphatidylinositol 4 phosphate 5 kinase 1α (PIP5K) is mainly localized in the cy...

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Published in:Biochemical and biophysical research communications 2013-09, Vol.439 (2), p.209-214
Main Authors: Chakrabarti, Rajarshi, Bhowmick, Debajit, Bhargava, Varsha, Bhar, Kaushik, Siddhanta, Anirban
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
AFFINITY
APOPTOSIS
CELL MEMBRANES
Cell Nucleolus - metabolism
COOLING PONDS
HEK293 Cells
Humans
PHOSPHATES
Phosphatidylinositol 4 phosphate 5 kinase 1α
Phosphotransferases (Alcohol Group Acceptor) - analysis
Phosphotransferases (Alcohol Group Acceptor) - metabolism
PolySUMOylation
RNF4
Small Ubiquitin-Related Modifier Proteins - metabolism
SUMO
SUMO-1 Protein - metabolism
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cited_by cdi_FETCH-LOGICAL-c384t-4abac5dd369001acc03ea81584cd086cd7e8c77b75800a771ab3837d2bd418b73
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container_title Biochemical and biophysical research communications
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creator Chakrabarti, Rajarshi
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description •Nuclear pool of PIP5K is SUMOylated.•Enhancement of SUMOylated nuclear PIP5K during apoptosis.•Nuclear PIP5K is modified by polySUMO-1 during apoptosis.•Nuclear PIP5K is modified by polySUMO-2 chain during apoptosis. Phosphatidylinositol 4 phosphate 5 kinase 1α (PIP5K) is mainly localized in the cytosol and plasma membrane. Studies have also indicated its prominent association with nuclear speckles. The exact nature of this nuclear pool of PIP5K is not clear. Using biochemical and microscopic techniques, we have demonstrated that the nuclear pool of PIP5K is modified by SUMO-1 in HEK-293 cells stably expressing PIP5K. Moreover, this SUMOylated pool of PIP5K increased during apoptosis. PolySUMO-2 chain conjugated PIP5K was detected by pull-down experiment using affinity-tagged RNF4, a polySUMO-2 binding protein, during late apoptosis.
doi_str_mv 10.1016/j.bbrc.2013.08.058
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subjects 60 APPLIED LIFE SCIENCES
AFFINITY
APOPTOSIS
CELL MEMBRANES
Cell Nucleolus - metabolism
COOLING PONDS
HEK293 Cells
Humans
PHOSPHATES
Phosphatidylinositol 4 phosphate 5 kinase 1α
Phosphotransferases (Alcohol Group Acceptor) - analysis
Phosphotransferases (Alcohol Group Acceptor) - metabolism
PolySUMOylation
RNF4
Small Ubiquitin-Related Modifier Proteins - metabolism
SUMO
SUMO-1 Protein - metabolism
title Nuclear pool of phosphatidylinositol 4 phosphate 5 kinase 1α is modified by polySUMO-2 during apoptosis
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