A flavone derivative from Sesbania sesban leaves and its cytotoxicity against murine leukemia P-388 cells

Sesbania sesban, locally named as Jayanti, is one of Indonesia plants belonging to Fabaceae family. This species is traditionally used by Indonesian people to cure digestive disorders, fever, or headache. Jayanti can grow well in tropical to subtropical region, such as in Asia and Africa. Based on l...

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Bibliographic Details
Main Authors: Hanhan, Dianhar, Syah Yana Maolana, Mujahidin Didin, Hakim Euis Holisotan, Dewi, Juliawaty Lia
Format: Conference Proceeding
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ALKALOIDS
ANALGESICS
ANTIOXIDANTS
Chromatography
Cytotoxicity
Fever
FLAVONOIDS
Headache
LEAVES
Leguminous plants
LEUKEMIA
Liquid chromatography
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
METHANOL
NMR
NUCLEAR MAGNETIC RESONANCE
Organic chemistry
PLANT TISSUES
PLANTS
Qualitative analysis
SAPONINS
TOXICITY
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Summary:Sesbania sesban, locally named as Jayanti, is one of Indonesia plants belonging to Fabaceae family. This species is traditionally used by Indonesian people to cure digestive disorders, fever, or headache. Jayanti can grow well in tropical to subtropical region, such as in Asia and Africa. Based on literature, qualitative analysis of the methanol extract of leaves of S. sesban showed that it contained flavonoids, alkaloids, saponins and glycosides. In addition, the activity assay of extracts of different tissues of this species showed antitumor, antimalarial, and antidiabetic activityies (leaves and seed extracts), antioxidants (flower extract), and analgesic (wood extract). Though the extracts of S. sesban parts showed interesting activities, chemical study of those extracts have not been widely reported. Therefore, the objective of this research was to isolate the secondary metabolites from methanol extract of leaves of S. sesban and to determine their cytotoxicity against murine leukemia P-388 cells. One compound has been obtained and identified as 3-hydroxy-4',7-dimethoxyflavone (1), a new isolated compound from nature. This compound was obtained through separation of methanol extract using various chromatographic techniques, such as vacuum liquid chromatography and radial chromatography. The structure elucidation of isolated compound was based on 1D NMR (1H-NMR and 13C-NMR) and 2D NMR (HMBC). The cytotoxicity of methanol extract and compound 1 against murine leukemia P-388 cells examined through MTT assay showed IC50 value of 60.04 μg/mL and 5.40 μg/mL, respectively.
ISSN:0094-243X
1551-7616
DOI:10.1063/1.4868831