Role of isothiocyanate conjugate of pterostilbene on the inhibition of MCF-7 cell proliferation and tumor growth in Ehrlich ascitic cell induced tumor bearing mice

• Published in: Experimental cell research 2014-01, Vol.320 (2), p.311-328
• Main Authors: Nikhil, Kumar, Sharan, Shruti, Chakraborty, Ajanta, Bodipati, Naganjaneyulu, Krishna Peddinti, Rama, Roy, Partha
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; Animals; Anti-cancer; APOPTOSIS; Biomedical research; Cancer therapies; Carcinoma, Ehrlich Tumor - pathology; CELL CYCLE; CELL PROLIFERATION; Cell Proliferation - drug effects; Conjugate; Cytotoxicity; DNA; Down-Regulation - drug effects; Drug Evaluation, Preclinical; Female; GROWTH; Hep G2 Cells; Humans; IN VITRO; INFLAMMATION; ISOTHIOCYANATES; Isothiocyanates - chemistry; Isothiocyanates - pharmacology; Male; MAMMARY GLANDS; MCF-7 Cells; MICE; Natural products; NEOPLASMS; PHOSPHORYLATION; Pterostilbene; Stilbenes - chemistry; Stilbenes - pharmacology; Studies; Tumor Burden - drug effects; Tumor Cells, Cultured; Tumors
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2013.10.015

Naturally occurring pterostilbene (PTER) and isothiocyanate (ITC) attract great attention due to their wide range of biological properties, including anti-cancer, anti-leukemic, anti-bacterial and anti-inflammatory activities. A novel class of hybrid compound synthesized by introducing an ITC moiety on PTER backbone was evaluated for its anti-cancer efficacy in hormone-dependent breast cancer cell line (MCF-7) in vitro and Ehrlich ascitic tumor bearing mice model in vivo. The novel hybrid molecule showed significant in vitro anti-cancer activity (IC50=25±0.38) when compared to reference compound PTER (IC50=65±0.42). The conjugate molecule induced both S and G2/M phase cell cycle arrest as indicated by flow cytometry analysis. In addition, the conjugate induced cell death was characterized by changes in cell morphology, DNA fragmentation, activation of caspase-9, release of cytochrome-c into cytosol and increased Bax: Bcl-2 ratio. The conjugate also suppressed the phosphorylation of Akt and ERK. The conjugate induced cell death was significantly increased in presence of A6730 (a potent Akt1/2 kinase inhibitor) and PD98059 (a specific ERK inhibitor). Moreover, the conjugated PTER inhibited tumor growth in Ehrlich ascitic cell induced tumor bearing mice as observed by reduction in tumor volume compared to untreated animals. Collectively, the pro-apoptotic effect of conjugate is mediated through the activation of caspases, and is correlated with the blockade of the Akt and ERK signaling pathways in MCF-7 cells. •Conjugate was prepared by appending isothiocyanate moiety on pterostilbene backbone.•Conjugate showed anticancer effects at comparatively lower dose than pterostilbene.•Conjugate caused blockage of the Akt and ERK signaling pathways in MCF-7 cells.•Conjugate significantly reduced solid tumor volume as compared to pterostilbene.