Oleanolic acid acetate inhibits atopic dermatitis and allergic contact dermatitis in a murine model

• Published in: Toxicology and applied pharmacology 2013-05, Vol.269 (1), p.72-80
• Main Authors: Choi, Jin Kyeong, Oh, Hyun-Mee, Lee, Soyoung, Park, Jin-Woo, Khang, Dongwoo, Lee, Seung Woong, Lee, Woo Song, Rho, Mun-Chual, Kim, Sang-Hyun
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ACETATES; Administration, Oral; Allergic contact dermatitis; Allergic diseases; Animals; Anti-Allergic Agents - administration & dosage; Anti-Allergic Agents - pharmacology; Antigens, Dermatophagoides; Atopic dermatitis; Biological and medical sciences; Cell Line; CYCLOSPORINE; Cytokines - metabolism; DERMATITIS; Dermatitis, Allergic Contact - genetics; Dermatitis, Allergic Contact - immunology; Dermatitis, Allergic Contact - pathology; Dermatitis, Allergic Contact - prevention & control; Dermatitis, Atopic - genetics; Dermatitis, Atopic - immunology; Dermatitis, Atopic - pathology; Dermatitis, Atopic - prevention & control; Dinitrochlorobenzene; Disease Models, Animal; ECZEMA; EOSINOPHILS; Eosinophils - drug effects; Eosinophils - immunology; Female; Gene Expression Regulation - drug effects; Humans; IMMUNE REACTIONS; Immunopathology; INFLAMMATION; Inflammation Mediators - metabolism; Keratinocyte; Keratinocytes - drug effects; Keratinocytes - immunology; Local Lymph Node Assay; LYMPH NODES; LYMPHOKINES; MAP Kinase Signaling System - drug effects; MAST CELLS; Mast Cells - drug effects; Mast Cells - immunology; Medical sciences; MICE; Mice, Inbred BALB C; NF-kappa B - metabolism; Oleanolic Acid - administration & dosage; Oleanolic Acid - pharmacology; Oleanolic acid acetate; SKIN; Skin - drug effects; Skin - immunology; Skin - pathology; Skin allergic diseases. Stinging insect allergies; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; Time Factors; Toxicology
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/j.taap.2013.03.001

Atopic dermatitis (AD) and allergic contact dermatitis (ACD) are common allergic and inflammatory skin diseases caused by a combination of eczema, scratching, pruritus, and cutaneous sensitization with allergens. This paper examines whether oleanolic acid acetate (OAA) modulates AD and ACD symptoms by using an existing AD model based on the repeated local exposure of mite extract (Dermatophagoides farinae extract, DFE) and 2,4-dinitrochlorobenzene to the ears of BALB/c mice. In addition, the paper uses a 2,4-dinitrofluorobenzene-sensitized local lymph node assay (LLNA) for the ACD model. The oral administration of OAA over a four-week period attenuated AD symptoms in terms of decreased skin lesions, epidermal thickness, the infiltration of immune cells (CD4+ cells, eosinophils, and mast cells), and serum IgE, IgG2a, and histamine levels. The gene expression of Th1, Th2, Th17, and Th22 cytokines was reduced by OAA in the lymph node and ear tissue, and the LLNA verified that OAA suppressed ACD. The oral administration of OAA over a three-day period attenuated ACD symptoms in terms of ear thickness, lymphocyte proliferation, and serum IgG2a levels. The gene expression of Th1, Th2, and Th17 cytokines was reduced by OAA in the thymus and ear tissue. Finally, to define the underlying mechanism, this paper uses a TNF-α/IFN-γ-activated human keratinocyte (HaCaT) model. OAA inhibited the expression of cytokines and chemokines through the downregulation of NF-κB and MAPKs in HaCaT cells. Taken together, the results indicate that OAA inhibited AD and ACD symptoms, suggesting that OAA may be effective in treating allergic skin disorders. [Display omitted] •OAA reduced both acute and chronic AD symptoms.•OAA had a controlling effect on the immune reaction for ACD.•The effect of OAA on allergic skin disorders was comparable to the cyclosporine A.•OAA might be a candidate for the treatment of allergic skin disorders.