MicroRNA-429 induces tumorigenesis of human non-small cell lung cancer cells and targets multiple tumor suppressor genes

• Published in: Biochemical and biophysical research communications 2014-07, Vol.450 (1), p.154-159
• Main Authors: Lang, Yaoguo, Xu, Shidong, Ma, Jianqun, Wu, Jun, Jin, Shi, Cao, Shoubo, Yu, Yan
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; Apoptosis - drug effects; Apoptosis - genetics; Carcinogenesis - drug effects; Carcinogenesis - genetics; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Cell Line, Tumor; CELL PROLIFERATION; COMPARATIVE EVALUATIONS; Gene Targeting - methods; GENES; Genes, Tumor Suppressor - drug effects; HUMAN POPULATIONS; Humans; INHIBITION; Lung Neoplasms - genetics; Lung Neoplasms - pathology; LUNGS; METASTASES; Metastasis; MicroRNAs - drug effects; MicroRNAs - genetics; MiR-429; Neoplasm Invasiveness; NEOPLASMS; Non-small cell lung cancer; PTEN; RASSF8; RESPIRATORY TRACT CELLS; RNA; THERAPY; TIMP2
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2014.05.084

•MiR-429 expression is upregulated in non-small cell lung cancer (NSCLC).•MiR-429 inhibits PTEN, RASSF8 and TIMP2 expression.•MiR-429 promotes metastasis and proliferation.•We report important regulatory mechanisms involved in NSCLC progression.•MiR-429 is a potential therapeutic target and diagnostic marker. Lung cancer is the major cause of cancer death globally. MicroRNAs are evolutionally conserved small noncoding RNAs that are critical for the regulation of gene expression. Aberrant expression of microRNA (miRNA) has been implicated in cancer initiation and progression. In this study, we demonstrated that the expression of miR-429 are often upregulated in non-small cell lung cancer (NSCLC) compared with normal lung tissues, and its expression level is also increased in NSCLC cell lines compared with normal lung cells. Overexpression of miR-429 in A549 NSCLC cells significantly promoted cell proliferation, migration and invasion, whereas inhibition of miR-429 inhibits these effects. Furthermore, we demonstrated that miR-429 down-regulates PTEN, RASSF8 and TIMP2 expression by directly targeting the 3′-untranslated region of these target genes. Taken together, our results suggest that miR-429 plays an important role in promoting the proliferation and metastasis of NSCLC cells and is a potential target for NSCLC therapy.