Inhibition of cell proliferation, migration and invasion of B16-F10 melanoma cells by α-mangostin

• Published in: Biochemical and biophysical research communications 2014-08, Vol.450 (4), p.1512-1517
• Main Authors: Beninati, Simone, Oliverio, Serafina, Cordella, Martina, Rossi, Stefania, Senatore, Cinzia, Liguori, Immacolata, Lentini, Alessandro, Piredda, Lucia, Tabolacci, Claudio
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ADHESION; AGGLOMERATION; Animals; Antimetastatic effects; Cell Line, Tumor; CELL PROLIFERATION; Cell Proliferation - drug effects; DRUGS; HUMAN POPULATIONS; IN VITRO; INHIBITION; MASS SPECTROSCOPY; Melanoma; Melanoma, Experimental - pathology; MELANOMAS; METASTASES; Mice; MOLECULES; Natural drugs; Neoplasm Invasiveness - prevention & control; Neoplasm Metastasis - prevention & control; REDUCTION; TUMOR CELLS; Xanthones - pharmacology; α-Mangostin
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2014.07.031

•We studied the anticancer potential of a new emerging molecule, α-mangostin (α-M).•We provide first evidences on the effects of α-M on transglutaminase activity.•We deeply examined the antimetastatic effects of α-M through many in vitro assays.•Proteomic analysis revealed that α-M promotes a reorganization at cellular level. In this study, we have evaluated the potential antineoplastic effects of α-mangostin (α-M), the most representative xanthone in Garcinia mangostana pericarp, on melanoma cell lines. This xanthone markedly inhibits the proliferation of high-metastatic B16-F10 melanoma cells. Furthermore, by deeply analyzing which steps in the metastatic process are influenced by xanthone it was observed that α-M strongly interferes with homotypic aggregation, adhesion, plasticity and invasion ability of B16-F10 cells, probably by the observed reduction of metalloproteinase-9 activity. The antiproliferative and antimetastatic properties of α-M have been established in human SK-MEL-28 and A375 melanoma cells. In order to identify pathways potentially involved in the antineoplastic properties of α-M, a comparative mass spectrometry proteomic approach was employed. These findings may improve our understanding of the molecular mechanisms underlying the anti-cancer effects of α-M on melanoma.