Safety analysis of a Russian phage cocktail: From MetaGenomic analysis to oral application in healthy human subjects

Abstract Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli / Pro...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2013-09, Vol.443 (2), p.187-196
Main Authors: McCallin, Shawna, Alam Sarker, Shafiqul, Barretto, Caroline, Sultana, Shamima, Berger, Bernard, Huq, Sayeda, Krause, Lutz, Bibiloni, Rodrigo, Schmitt, Bertrand, Reuteler, Gloria, Brüssow, Harald
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Administration, Oral
BACTERIOPHAGES
Bacteriophages - classification
Bacteriophages - genetics
Bacteriophages - ultrastructure
Biological Therapy - adverse effects
Biological Therapy - methods
CLINICAL TRIALS
CONTIGS
EASTERN EUROPE
ELECTRON MICROSCOPY
ESCHERICHIA COLI
Escherichia coli Infections - therapy
Fecal microbiota
GENES
Gram-Negative Bacteria - classification
Gram-Negative Bacteria - virology
Human phage therapy
Humans
Infectious Disease
Metagenomics
Metagenomics - methods
Microscopy, Electron, Transmission
Myoviridae
Myoviridae - classification
Myoviridae - genetics
Myoviridae - ultrastructure
Myovirus
Podoviridae
Podoviridae - classification
Podoviridae - genetics
Podoviridae - ultrastructure
PROTEUS
Proteus Infections - therapy
Russia
Safety
SAFETY ANALYSIS
Siphoviridae
Siphoviridae - classification
Siphoviridae - genetics
Siphoviridae - ultrastructure
THERAPY
Treatment Outcome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Phage therapy has a long tradition in Eastern Europe, where preparations are comprised of complex phage cocktails whose compositions have not been described. We investigated the composition of a phage cocktail from the Russian pharmaceutical company Microgen targeting Escherichia coli / Proteus infections. Electron microscopy identified six phage types, with numerically T7-like phages dominating over T4-like phages. A metagenomic approach using taxonomical classification, reference mapping and de novo assembly identified 18 distinct phage types, including 7 genera of Podoviridae, 2 established and 2 proposed genera of Myoviridae, and 2 genera of Siphoviridae. De novo assembly yielded 7 contigs greater than 30 kb, including a 147-kb Myovirus genome and a 42-kb genome of a potentially new phage. Bioinformatic analysis did not reveal undesired genes and a small human volunteer trial did not associate adverse effects with oral phage exposure.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2013.05.022