10-oxo-12(Z)-octadecenoic acid, a linoleic acid metabolite produced by gut lactic acid bacteria, potently activates PPARγ and stimulates adipogenesis

• Published in: Biochemical and biophysical research communications 2015-04, Vol.459 (4), p.597-603
• Main Authors: Goto, Tsuyoshi, Kim, Young-Il, Furuzono, Tomoya, Takahashi, Nobuyuki, Yamakuni, Kanae, Yang, Ha-Eun, Li, Yongjia, Ohue, Ryuji, Nomura, Wataru, Sugawara, Tatsuya, Yu, Rina, Kitamura, Nahoko, Park, Si-Bum, Kishino, Shigenobu, Ogawa, Jun, Kawada, Teruo
• Format: Article
• Language: English
• Subjects: 10-oxo-12(Z)-octadecenoic acid; 60 APPLIED LIFE SCIENCES; Adipocyte differentiation; Adipogenesis - drug effects; Animals; BACTERIA; Energy Metabolism; Fatty acid; GLUCOSE; Gut lactic acid bacteria; INSULIN; LACTIC ACID; Lactobacillus - metabolism; LINOLEIC ACID; Linoleic Acids - biosynthesis; Linoleic Acids - pharmacology; LIPASES; LIPOPROTEINS; LUCIFERASE; METABOLIC DISEASES; METABOLISM; Mice; NIH 3T3 Cells; Obesity; OCTADECANOIC ACID; OLEIC ACID; Polymerase Chain Reaction; PPAR gamma - metabolism; PPARγ; RECEPTORS; UPTAKE
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2015.02.154

Our previous study has shown that gut lactic acid bacteria generate various kinds of fatty acids from polyunsaturated fatty acids such as linoleic acid (LA). In this study, we investigated the effects of LA and LA-derived fatty acids on the activation of peroxisome proliferator-activated receptors (PPARs) which regulate whole-body energy metabolism. None of the fatty acids activated PPARδ, whereas almost all activated PPARα in luciferase assays. Two fatty acids potently activated PPARγ, a master regulator of adipocyte differentiation, with 10-oxo-12(Z)-octadecenoic acid (KetoA) having the most potency. In 3T3-L1 cells, KetoA induced adipocyte differentiation via the activation of PPARγ, and increased adiponectin production and insulin-stimulated glucose uptake. These findings suggest that fatty acids, including KetoA, generated in gut by lactic acid bacteria may be involved in the regulation of host energy metabolism. •Most LA-derived fatty acids from gut lactic acid bacteria potently activated PPARα.•Among tested fatty acids, KetoA and KetoC significantly activated PPARγ.•KetoA induced adipocyte differentiation via the activation of PPARγ.•KetoA enhanced adiponectin production and glucose uptake during adipogenesis.