Flavonoids casticin and chrysosplenol D from Artemisia annua L. inhibit inflammation in vitro and in vivo

• Published in: Toxicology and applied pharmacology 2015-08, Vol.286 (3), p.151-158
• Main Authors: Li, Yu-Jie, Guo, Yan, Yang, Qing, Weng, Xiao-Gang, Yang, Lan, Wang, Ya-Jie, Chen, Ying, Zhang, Dong, Li, Qi, Liu, Xu-Cen, Kan, Xiao-Xi, Chen, Xi, Zhu, Xiao-Xin, Kmoníèková, Eva, Zídek, Zdenìk
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; Animals; Artemisia annua; Artemisia annua L; AUDITORY ORGANS; Casticin; Chrysosplenol D; Croton; Dermatitis - drug therapy; Dermatitis - pathology; DEXAMETHASONE; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - isolation & purification; Drugs, Chinese Herbal - therapeutic use; EDEMA; Edema - drug therapy; Edema - pathology; Flavones - isolation & purification; Flavones - therapeutic use; FLAVONOIDS; Flavonoids - isolation & purification; Flavonoids - therapeutic use; HT29 Cells; Human Umbilical Vein Endothelial Cells; Humans; IN VITRO; IN VIVO; INFLAMMATION; Inflammation - drug therapy; Inflammation - pathology; INTRAPERITONEAL INJECTION; MACROPHAGES; Male; MICE; Mice, Inbred ICR; PHOSPHORYLATION; TRIGLYCERIDES; VEGETABLE OILS
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/j.taap.2015.04.005

The aim of our experiments was to investigate the anti-inflammatory properties of casticin and chrysosplenol D, two flavonoids present in Artemisia annua L. Topical inflammation was induced in ICR mice using croton oil. Mice were then treated with casticin or chrysosplenol D. Cutaneous histological changes and edema were assessed. ICR mice were intragastrically administrated with casticin or chrysosplenol D followed by intraperitoneal injection of lipopolysaccharide (LPS). Mouse Raw264.7 macrophage cells were incubated with casticin or chrysosplenol D. Intracellular phosphorylation was detected, and migration was assessed by trans-well assay. HT-29/NFκB-luc cells were incubated with casticin or chrysosplenol D in the presence or absence of LPS, and NF-κB activation was quantified. In mice, administration of casticin (0.5, 1 and 1.5μmol/cm2) and chrysosplenol D (1 and 1.5μmol/cm2) inhibited croton oil-induced ear edema (casticin: 29.39–64.95%; chrysosplenol D: 37.76–65.89%, all P