Combined fluorescence and phosphorescence lifetime imaging

We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and p...

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Bibliographic Details
Published in:Applied physics letters 2016-02, Vol.108 (9)
Main Authors: Shcheslavskiy, V. I., Neubauer, A., Bukowiecki, R., Dinter, F., Becker, W.
Format: Article
Language:English
Subjects:
Applied physics
BIOMEDICAL RADIOGRAPHY
CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS
FLUORESCENCE
KIDNEYS
LASERS
LIFETIME
PHOSPHORESCENCE
PHOTONS
Pulsed lasers
PULSES
Recording
SIGNALS
Time correlation functions
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Summary:We present a lifetime imaging technique that simultaneously records the fluorescence and phosphorescence lifetime images in confocal laser scanning systems. It is based on modulating a high-frequency pulsed laser synchronously with the pixel clock of the scanner, and recording the fluorescence and phosphorescence signals by multidimensional time-correlated single photon counting board. We demonstrate our technique on the recording of the fluorescence/phosphorescence lifetime images of human embryonic kidney cells at different environmental conditions.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.4943265