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miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2
miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched...
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Published in: | Biochemical and biophysical research communications 2016-08, Vol.476 (4), p.607-613 |
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creator | Zhang, Yunda Xu, Guoxing Liu, Gang Ye, Yongzhi Zhang, Chuankai Fan, Chuannan Wang, Haibin Cai, Huali Xiao, Rui Huang, Zhengjie Luo, Qi |
description | miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched adjacent non-neoplastic tissues. In addition, the expression of miR-411-5p was also lower in breast cancer cell lines in contrast with MCF-10A. Moreover, we investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR-411-5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR-411-5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR-411-5p related mechanism in the progression of breast cancer and, miR-411-5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy.
•miR-411-5p is downregulated in breast cancer tissues and cell lines.•miR-411-5p inhibits breast cancer cells growth, migration and invasion in vitro.•GRB2 is a direct target of miR-411-5p in breast cancer.•GRB2 is overexpressed in breast cancer and associates with disease outcome.•miR-411-5p suppresses breast cancer progression though GRB2-SOS-Ras pathway. |
doi_str_mv | 10.1016/j.bbrc.2016.06.006 |
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•miR-411-5p is downregulated in breast cancer tissues and cell lines.•miR-411-5p inhibits breast cancer cells growth, migration and invasion in vitro.•GRB2 is a direct target of miR-411-5p in breast cancer.•GRB2 is overexpressed in breast cancer and associates with disease outcome.•miR-411-5p suppresses breast cancer progression though GRB2-SOS-Ras pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2016.06.006</identifier><identifier>PMID: 27264952</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>3' Untranslated Regions ; 60 APPLIED LIFE SCIENCES ; ANIMAL TISSUES ; Breast cancer ; breast neoplasms ; Breast Neoplasms - genetics ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Cell Line, Tumor ; Cell Movement - genetics ; CELL PROLIFERATION ; Cell Proliferation - genetics ; Disease Progression ; Down-Regulation ; Female ; Gene Expression ; Gene Silencing ; GRB2 ; GRB2 Adaptor Protein - genetics ; GRB2 Adaptor Protein - metabolism ; human diseases ; Humans ; IN VITRO ; MAMMARY GLANDS ; MCF-7 Cells ; METASTASES ; Metastasis ; MicroRNAs - genetics ; miR-411-5p ; Neoplasm Invasiveness - genetics ; Neoplasm Metastasis - genetics ; NEOPLASMS ; Proliferation ; ras Proteins - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Neoplasm - genetics ; RNA, Neoplasm - metabolism ; Signal Transduction - genetics</subject><ispartof>Biochemical and biophysical research communications, 2016-08, Vol.476 (4), p.607-613</ispartof><rights>2016 Elsevier Inc.</rights><rights>Copyright © 2016 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-f9c9fd4b5743d01e584b6812d1e07e31ac3b5840285863b183aaf393c07b1ba53</citedby><cites>FETCH-LOGICAL-c408t-f9c9fd4b5743d01e584b6812d1e07e31ac3b5840285863b183aaf393c07b1ba53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27264952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/22606141$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yunda</creatorcontrib><creatorcontrib>Xu, Guoxing</creatorcontrib><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Ye, Yongzhi</creatorcontrib><creatorcontrib>Zhang, Chuankai</creatorcontrib><creatorcontrib>Fan, Chuannan</creatorcontrib><creatorcontrib>Wang, Haibin</creatorcontrib><creatorcontrib>Cai, Huali</creatorcontrib><creatorcontrib>Xiao, Rui</creatorcontrib><creatorcontrib>Huang, Zhengjie</creatorcontrib><creatorcontrib>Luo, Qi</creatorcontrib><title>miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched adjacent non-neoplastic tissues. In addition, the expression of miR-411-5p was also lower in breast cancer cell lines in contrast with MCF-10A. Moreover, we investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR-411-5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR-411-5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR-411-5p related mechanism in the progression of breast cancer and, miR-411-5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy.
•miR-411-5p is downregulated in breast cancer tissues and cell lines.•miR-411-5p inhibits breast cancer cells growth, migration and invasion in vitro.•GRB2 is a direct target of miR-411-5p in breast cancer.•GRB2 is overexpressed in breast cancer and associates with disease outcome.•miR-411-5p suppresses breast cancer progression though GRB2-SOS-Ras pathway.</description><subject>3' Untranslated Regions</subject><subject>60 APPLIED LIFE SCIENCES</subject><subject>ANIMAL TISSUES</subject><subject>Breast cancer</subject><subject>breast neoplasms</subject><subject>Breast Neoplasms - genetics</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>CELL PROLIFERATION</subject><subject>Cell Proliferation - genetics</subject><subject>Disease Progression</subject><subject>Down-Regulation</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Silencing</subject><subject>GRB2</subject><subject>GRB2 Adaptor Protein - genetics</subject><subject>GRB2 Adaptor Protein - metabolism</subject><subject>human diseases</subject><subject>Humans</subject><subject>IN VITRO</subject><subject>MAMMARY GLANDS</subject><subject>MCF-7 Cells</subject><subject>METASTASES</subject><subject>Metastasis</subject><subject>MicroRNAs - genetics</subject><subject>miR-411-5p</subject><subject>Neoplasm Invasiveness - genetics</subject><subject>Neoplasm Metastasis - genetics</subject><subject>NEOPLASMS</subject><subject>Proliferation</subject><subject>ras Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Neoplasm - genetics</subject><subject>RNA, Neoplasm - metabolism</subject><subject>Signal Transduction - genetics</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kVGL1DAQgIMo3nr6B3zQgC--dJ1J07QBX_TQUzgQTg_0KSTpdC_Ltt1Lsgf-e1N6-igMJBm-GWa-MPYSYYuA6t1-61z0W1HuWygB6hHbIGioBIJ8zDZQUpXQ-POMPUtpD4AolX7KzkQrlNSN2LBfY7iuJGLVHHmYboMLOfFjnA9hoGhzmCdup56PlG0qERKfB-4ilRf3dvIUuafDgd8Hy7ONO8ph2vHL64_iOXsy2EOiFw_nObv5_OnHxZfq6tvl14sPV5WX0OVq0F4PvXRNK-sekJpOOtWh6JGgpRqtr13JgeiaTtUOu9raoda1h9ahs019zt6sfeeUg0k-ZPK3fp4m8tkIoUChxEK9Xamy292JUjZjSMvkdqL5lAx2IGQri7iCihX1cU4p0mCOMYw2_jYIZhFv9mYRbxbxBkqAKkWvHvqf3Ej9v5K_pgvwegUGOxu7iyGZm-9Lh_IrWimlC_F-JajYug8Ul2WoKO5DXHbp5_C_Cf4A-qGa8A</recordid><startdate>20160805</startdate><enddate>20160805</enddate><creator>Zhang, Yunda</creator><creator>Xu, Guoxing</creator><creator>Liu, Gang</creator><creator>Ye, Yongzhi</creator><creator>Zhang, Chuankai</creator><creator>Fan, Chuannan</creator><creator>Wang, Haibin</creator><creator>Cai, Huali</creator><creator>Xiao, Rui</creator><creator>Huang, Zhengjie</creator><creator>Luo, Qi</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20160805</creationdate><title>miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2</title><author>Zhang, Yunda ; Xu, Guoxing ; Liu, Gang ; Ye, Yongzhi ; Zhang, Chuankai ; Fan, Chuannan ; Wang, Haibin ; Cai, Huali ; Xiao, Rui ; Huang, Zhengjie ; Luo, Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-f9c9fd4b5743d01e584b6812d1e07e31ac3b5840285863b183aaf393c07b1ba53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>3' Untranslated Regions</topic><topic>60 APPLIED LIFE SCIENCES</topic><topic>ANIMAL TISSUES</topic><topic>Breast cancer</topic><topic>breast neoplasms</topic><topic>Breast Neoplasms - genetics</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>CELL PROLIFERATION</topic><topic>Cell Proliferation - genetics</topic><topic>Disease Progression</topic><topic>Down-Regulation</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gene Silencing</topic><topic>GRB2</topic><topic>GRB2 Adaptor Protein - genetics</topic><topic>GRB2 Adaptor Protein - metabolism</topic><topic>human diseases</topic><topic>Humans</topic><topic>IN VITRO</topic><topic>MAMMARY GLANDS</topic><topic>MCF-7 Cells</topic><topic>METASTASES</topic><topic>Metastasis</topic><topic>MicroRNAs - genetics</topic><topic>miR-411-5p</topic><topic>Neoplasm Invasiveness - genetics</topic><topic>Neoplasm Metastasis - genetics</topic><topic>NEOPLASMS</topic><topic>Proliferation</topic><topic>ras Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Neoplasm - genetics</topic><topic>RNA, Neoplasm - metabolism</topic><topic>Signal Transduction - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yunda</creatorcontrib><creatorcontrib>Xu, Guoxing</creatorcontrib><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Ye, Yongzhi</creatorcontrib><creatorcontrib>Zhang, Chuankai</creatorcontrib><creatorcontrib>Fan, Chuannan</creatorcontrib><creatorcontrib>Wang, Haibin</creatorcontrib><creatorcontrib>Cai, Huali</creatorcontrib><creatorcontrib>Xiao, Rui</creatorcontrib><creatorcontrib>Huang, Zhengjie</creatorcontrib><creatorcontrib>Luo, Qi</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yunda</au><au>Xu, Guoxing</au><au>Liu, Gang</au><au>Ye, Yongzhi</au><au>Zhang, Chuankai</au><au>Fan, Chuannan</au><au>Wang, Haibin</au><au>Cai, Huali</au><au>Xiao, Rui</au><au>Huang, Zhengjie</au><au>Luo, Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2016-08-05</date><risdate>2016</risdate><volume>476</volume><issue>4</issue><spage>607</spage><epage>613</epage><pages>607-613</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>miR-411-5p (previously called miR-411) is severely involved in human diseases, however, the relationship between miR-411-5p and breast cancer has not been investigated thoroughly. Here, we found that the expression of miR-411-5p was downregulated in breast cancer tissues compared with their matched adjacent non-neoplastic tissues. In addition, the expression of miR-411-5p was also lower in breast cancer cell lines in contrast with MCF-10A. Moreover, we investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation. Ectopic expression of miR-411-5p suppressed the breast cancer cell proliferation, migration and invasion while low expression of miR-411-5p exhibited the opposite effect. Furthermore, GRB2 was demonstrated to be significantly overexpressed in breast cancer tissues compared with normal tissues, and low expression of GRB2 had a longer overall survival compared with high expression of GRB2 in breast cancer. In general, our study shed light on the miR-411-5p related mechanism in the progression of breast cancer and, miR-411-5p/GRB2/Ras axis is potential to be molecular target for breast cancer therapy.
•miR-411-5p is downregulated in breast cancer tissues and cell lines.•miR-411-5p inhibits breast cancer cells growth, migration and invasion in vitro.•GRB2 is a direct target of miR-411-5p in breast cancer.•GRB2 is overexpressed in breast cancer and associates with disease outcome.•miR-411-5p suppresses breast cancer progression though GRB2-SOS-Ras pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>27264952</pmid><doi>10.1016/j.bbrc.2016.06.006</doi><tpages>7</tpages></addata></record> |
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subjects | 3' Untranslated Regions 60 APPLIED LIFE SCIENCES ANIMAL TISSUES Breast cancer breast neoplasms Breast Neoplasms - genetics Breast Neoplasms - metabolism Breast Neoplasms - pathology Cell Line, Tumor Cell Movement - genetics CELL PROLIFERATION Cell Proliferation - genetics Disease Progression Down-Regulation Female Gene Expression Gene Silencing GRB2 GRB2 Adaptor Protein - genetics GRB2 Adaptor Protein - metabolism human diseases Humans IN VITRO MAMMARY GLANDS MCF-7 Cells METASTASES Metastasis MicroRNAs - genetics miR-411-5p Neoplasm Invasiveness - genetics Neoplasm Metastasis - genetics NEOPLASMS Proliferation ras Proteins - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Neoplasm - genetics RNA, Neoplasm - metabolism Signal Transduction - genetics |
title | miR-411-5p inhibits proliferation and metastasis of breast cancer cell via targeting GRB2 |
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