Bioreactor with Ipomoea hederifolia adventitious roots and its endophyte Cladosporium cladosporioides for textile dye degradation

• Published in: Environmental research 2016-04, Vol.146, p.340-349
• Main Authors: Patil, Swapnil M., Chandanshive, Vishal V., Rane, Niraj R., Khandare, Rahul V., Watharkar, Anuprita D., Govindwar, Sanjay P.
• Format: Article
• Language: English
• Subjects: Biodegradation, Environmental; BIOLOGICAL PATHWAYS; BIOREACTORS; Cladding; Cladosporium - metabolism; Cladosporium cladosporioides; Coloring Agents - metabolism; Decoloring; Decolorization; Dyes; ECOLOGICAL CONCENTRATION; Endophytes - metabolism; Endophytic fungus; Environmental Pollutants - metabolism; Environmental Restoration and Remediation - methods; ENVIRONMENTAL SCIENCES; FOURIER TRANSFORMATION; FUNGI; GAS CHROMATOGRAPHY; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; Ipomoea - metabolism; Ipomoea hederifolia; Laccase; MASS SPECTROSCOPY; Niobium; PEROXIDASES; Phytoremediation; Plant Roots - metabolism; RIBOFLAVIN; Roots; Textile Industry; TYROSINASE; Waste Disposal, Fluid - methods
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2016.01.019

In vitro grown untransformed adventitious roots (AR) culture of Ipomoea hederifolia and its endophytic fungus (EF) Cladosporium cladosporioides decolorized Navy Blue HE2R (NB-HE2R) at a concentration of 20 ppm up to 83.3 and 65%, respectively within 96h. Whereas the AR-EF consortium decolorized the dye more efficiently and gave 97% removal within 36h. Significant inductions in the enzyme activities of lignin peroxidase, tyrosinase and laccase were observed in roots, while enzymes like tyrosinase, laccase and riboflavin reductase activities were induced in EF. Metabolites of dye were analyzed using UV—vis spectroscopy, FTIR and gas chromatography-mass spectrometry. Possible metabolic pathways of NB-HE2R were proposed with AR, EF and AR-EF systems independently. Looking at the superior efficacy of AR-EF system, a rhizoreactor was developed for the treatment of NB-HE2R at a concentration of 1000 ppm. Control reactor systems with independently grown AR and EF gave 94 and 85% NB-HE2R removal, respectively within 36h. The AR-EF rhizoreactor, however, gave 97% decolorization. The endophyte colonization additionally increased root and shoot lengths of candidate plants through mutualism. Combined bioreactor strategies can be effectively used for future eco-friendly remediation purposes. [Display omitted] •Endophytic fungus on Ipomoea hederifolia promotes root growth and shoot development•Endophytic Cladosporium cladosporioides synergistically degrade Navy Blue-HE2R dye•Endophyte colonized I. hederifolia roots proved superior in dye decolorization•Dye stress and toxicity was efficiently dealt by root-endophyte consortium•Root-endophyte consortium can be used as a sustainable remediation strategy