Small-angle X-ray scattering study of the influence of solvent replacement (from H2O to D2O) on the initial crystallization stage of tetragonal lysozyme

The composition of lysozyme solutions in D 2 O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric specie...

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Bibliographic Details
Published in:Crystallography reports 2017-11, Vol.62 (6), p.837-842
Main Authors: Boikova, A. S., D’yakova, Yu. A., Il’ina, K. B., Konarev, P. V., Kryukova, A. E., Marchenkova, M. A., Blagov, A. E., Pisarevskii, Yu. V., Koval’chuk, M. V.
Format: Article
Language:English
Subjects:
COMPARATIVE EVALUATIONS
Concentration (composition)
CONCENTRATION RATIO
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
CRYSTALLIZATION
Crystallography and Scattering Methods
CRYSTALS
Diffraction and Scattering of Ionizing Radiations
HEAVY WATER
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
Light water
LYSOZYME
MONOMERS
Physics
Physics and Astronomy
SMALL ANGLE SCATTERING
Small angle X ray scattering
Solvents
SYNCHROTRON RADIATION
X-RAY DIFFRACTION
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Summary:The composition of lysozyme solutions in D 2 O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with H 2 O used as a solvent has shown that the replacement of light water with heavy one leads to increase of octamer volume fraction in solution.
ISSN:1063-7745
1562-689X
DOI:10.1134/S1063774517060074