Submicrometer Emitter ESI Tips for Native Mass Spectrometry of Membrane Proteins in Ionic and Nonionic Detergents

Native mass spectrometry (native-MS) of membrane proteins typically requires a detergent screening protocol, protein solubilization in the preferred detergent, followed by protein liberation from the micelle by collisional activation. Here, submicrometer nano-ESI emitter tips are used for native-MS...

Full description

Saved in:
Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2018-01, Vol.29 (1), p.203-206
Main Authors: Susa, Anna C., Lippens, Jennifer L., Xia, Zijie, Loo, Joseph A., Campuzano, Iain D. G., Williams, Evan R.
Format: Article
Language:English
Subjects:
Ammonium acetate
Analytical Chemistry
Application Note
Aqueous solutions
Bioinformatics
Biotechnology
Charge distribution
CHARGE STATES
Chemistry
Chemistry and Materials Science
Clusters
COMPARATIVE EVALUATIONS
Detergents
Emitters
INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY
ION PAIRS
Ions
Mass spectrometry
MASS SPECTROSCOPY
MEMBRANE PROTEINS
Organic Chemistry
Proteins
Proteomics
Scientific imaging
SIGNALS
Solubilization
SOLUTIONS
Spectroscopy
Tips
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Native mass spectrometry (native-MS) of membrane proteins typically requires a detergent screening protocol, protein solubilization in the preferred detergent, followed by protein liberation from the micelle by collisional activation. Here, submicrometer nano-ESI emitter tips are used for native-MS of membrane proteins solubilized in both nonionic and ionic detergent solutions. With the submicrometer nano-ESI emitter tips, resolved charge-state distributions of membrane protein ions are obtained from a 150 mM NaCl, 25 mM Tris-HCl with 1.1% octyl glucoside solution. The relative abundances of NaCl and detergent cluster ions at high m  / z are significantly reduced with the submicrometer emitters compared with larger nano-ESI emitters that are commonly used. This technique is beneficial for significantly decreasing the abundances (by two to three orders of magnitude compared with the larger tip size: 1.6 μm) of detergent cluster ions formed from aqueous ammonium acetate solutions containing detergents that can overlap with the membrane protein ion signal. Resolved charge-state distributions of membrane protein ions from aqueous ammonium acetate solutions containing ionic detergents were obtained with the submicrometer nano-ESI emitters; this is the first report of native-MS of membrane proteins solubilized by ionic detergents. Graphical Abstract
ISSN:1044-0305
1879-1123
DOI:10.1007/s13361-017-1793-x