Optical Methods for the Analysis of the Temoprofin Photosensitizer Distribution Between Serum Proteins and Methyl-β-Cyclodextrin Nanocarriers in Blood Serum

Various optical methods for the analysis of the processes leading to temoporfin photosensitizer distribution between supramolecular nanosized inclusion complexes derived from β−cyclodextrins and blood serum proteins were examined. Methods involving induced circular dichroism, fluorescence anisotropy...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2018-01, Vol.84 (6), p.1030-1036
Main Authors: Yakavets, I. V., Yankovsky, I. V., Khludeyev, I. I., Lassalle, H.P., Bezdetnaya, L. N., Zorin, V. P.
Format: Article
Language:English
Subjects:
Analytical Chemistry
ANISOTROPY
Atomic/Molecular Structure and Spectra
Blood
BLOOD SERUM
CHROMATOGRAPHY
CLATHRATES
Cyclodextrins
DICHROISM
EQUILIBRIUM
Excitation spectra
Feasibility studies
FLUORESCENCE
GELS
Inclusion complexes
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
Life Sciences
NANOSCIENCE AND NANOTECHNOLOGY
NANOSTRUCTURES
OLIGOSACCHARIDES
Optical properties
Optics
Physics
Physics and Astronomy
Proteins
Serum proteins
ULTRACENTRIFUGATION
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Summary:Various optical methods for the analysis of the processes leading to temoporfin photosensitizer distribution between supramolecular nanosized inclusion complexes derived from β−cyclodextrins and blood serum proteins were examined. Methods involving induced circular dichroism, fluorescence anisotropy, and the variability of the shape of the photosensitizer fluorescence excitation spectra were compared with traditional methods such as gel chromatography and ultracentrifugation. The feasibility of using the photosensitizer optical characteristics for analyzing both equilibrium and kinetic processes of photosensitizer distribution in blood was demonstrated. The main advantages and limitations of these approaches in in vitro experiments were described.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-018-0582-z