Combined Impact of Gamma and Laser Radiation on Peripheral Blood of Rats in vivo

• Published in: Journal of applied spectroscopy 2017-11, Vol.84 (5), p.796-803
• Main Authors: Zalesskaya, G. A., Batay, L. E., Koshlan, I. V., Nasek, V. M., Zilberman, R. D., Milevich, T. I., Govorun, R. D., Koshlan, N. A., Blaga, P.
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ABSORPTION SPECTRA; Analytical Chemistry; Animal experimentation; Antioxidants; Antioxidants (Nutrients); Atomic/Molecular Structure and Spectra; Blood; ENZYMES; ERYTHROCYTES; Gamma rays; Group dynamics; IN VIVO; Ionizing radiation; IONIZING RADIATIONS; IRRADIATION; Laser beams; LASER RADIATION; Lasers; Leukocytes; LYMPHOCYTES; Optical radiation; Physics; Physics and Astronomy; Rapid prototyping; RATS; SUPEROXIDE DISMUTASE; Superoxides; VEINS
• ISSN: 0021-9037; 1573-8647
• DOI: 10.1007/s10812-017-0547-7

The impact of γ radiation of 137 Сs (doses of 1 and 3 Gy), low-intensity laser radiation (λ = 670 nm, 5.3 or 10.6 J/cm 2 ) as well as the influence of consecutive laser and γ radiation on peripheral blood and blood cells (erythrocytes, leukocytes, lymphocytes, granulocytes) were studied by analyzing the number of blood cells, blood absorption spectra, and activity of antioxidant defense enzymes. Two series of experiments were performed on four groups of rats. The rats of the control group (group 1) were not exposed to γ or laser radiation. In the experimental groups, single irradiation of the whole body of rats with γ radiation (group 2), three- or four-day over-vein irradiation of blood in the tail vein by low-intensity laser radiation (group 3), and successive three- or four-day irradiation of blood by laser and then a single irradiation of the whole body with γ radiation (group 4) were performed. It was shown that changes of the blood cell content in the experimental groups are accompanied by changes in the spectral characteristics of the blood and the activity of antioxidant defense enzymes. The radioprotective effect of low-intensity laser radiation is manifested as an increase in the average number of leukocytes and lymphocytes in the group as compared with the postradiation, as well as an increase in the activity of antioxidant protection enzymes. The possibility of using low-intensity optical radiation for correction of hematological disorders caused by ionizing radiation is discussed.