Study of the Osteoindictive Properties of Protein-Modified Polylactide Scaffolds

• Published in: Bulletin of experimental biology and medicine 2019-05, Vol.167 (1), p.164-168
• Main Authors: Nashchekina, Yu. A., Alexandrova, S. A., Nikonov, P. О., Ivankova, E. I., Yudin, V. E., Blinova, M. I., Mikhailova, N. A.
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ALIZARIN; ALKALINE PHOSPHATASE; Alkaline Phosphatase - metabolism; Analysis; Animals; Biomedical and Life Sciences; Biomedicine; BONE MARROW; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; CALCIUM; Cell Biology; Cell culture; CELL CULTURES; Cell Differentiation - physiology; Cells, Cultured; COLLAGEN; Collagen (type I); Collagen Type I - metabolism; Electron microscopy; FIBRIN; Fibrin - metabolism; IN VITRO; Internal Medicine; Laboratory Medicine; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - physiology; Mesenchyme; Microscopy; Nodules; Osteocalcin - metabolism; Osteogenesis; Osteogenesis - physiology; Pathology; Phosphatase; Phosphatases; Polyesters - chemistry; Polylactic acid; Rabbits; SCANNING ELECTRON MICROSCOPY; SPECTROPHOTOMETRY; Stem cells; Stromal cells; Tissue Engineering - methods; Tissue Scaffolds - chemistry; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-019-04483-3

Bone marrow mesenchymal stromal cells are multipotent and can differentiate into cells of various tissues, which determines their high importance for clinical application. We performed an in vitro study of the osteogenic potential of mesenchymal stromal cells cultured on intact polylactide scaffolds or scaffolds modified with collagen I or fibrin. Scanning electron microscopy showed that the cells formed osteogenic nodules or osteogenic nodules on both intact and fibrin-modified polylactide scaffolds. Spectrophotometric detection of alkaline phosphatase activity on days 7 and 11 showed that mesenchymal stromal cell grown on intact polylactide scaffolds and on scaffolds modified with collagen type I or fibrin more intensively synthesized alkaline phosphatase than in the control (culture plastic). This dependence increases in the presence of osteogenic differentiation factors in the medium. After long-term culturing (4 weeks), the presence of calcium deposits detected by alizarin red staining confirmed the osteoinductive properties of intact and protein-modified polylactide scaffolds. These findings suggest that polylactide scaffolds and collagen I increase the osteogenic differentiation potential of mesenchymal stromal cells.