Polarized Fluorescence in NADH Two-Photon Excited by Femtosecond Laser Pulses in the Wavelength Range of 720–780 nm

The decay of polarized fluorescence in coenzyme NADH in aqueous-methanol solution under two-photon excitation by femtosecond laser pulses within the spectral range of 720–780 nm has been studied. Molecular fluorescence parameters: decay times τ 1 and τ 2 , rotational diffusion time τ rot , and pre-e...

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Bibliographic Details
Published in:Technical physics letters 2019-07, Vol.45 (7), p.672-674
Main Authors: Sasin, M. E., Gorbunova, I. A., Bezverkhnii, N. O., Beltukov, Y. M., Vasyutinskii, O. S., Rubayo-Soneira, J.
Format: Article
Language:English
Subjects:
AQUEOUS SOLUTIONS
Classical and Continuum Physics
COENZYMES
CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY
Decay
DIFFUSION
EXCITATION
Femtosecond pulses
FLUORESCENCE
LASER RADIATION
METHANOL
Nicotinamide adenine dinucleotide
PHOTONS
Physics
Physics and Astronomy
PULSES
SPECTROSCOPY
WAVELENGTHS
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Summary:The decay of polarized fluorescence in coenzyme NADH in aqueous-methanol solution under two-photon excitation by femtosecond laser pulses within the spectral range of 720–780 nm has been studied. Molecular fluorescence parameters: decay times τ 1 and τ 2 , rotational diffusion time τ rot , and pre-exponential factor ratio a 2 / a 1 have been determined from experiment. The results obtained have been compared with those reported recently in NADH in aqueous solution.
ISSN:1063-7850
1090-6533
DOI:10.1134/S1063785019070101