CNT-Based Label-Free Electrochemical Sensing of Native DNA with Allele Single Nucleotide Polymorphism

A label-free high-performance electrochemical sensing of single nucleotide polymorphism in native genomic DNA samples from cancer tissues, in oncogene-specific complementary DNA and in amplicons has been carried out on bundles of few-walled carboxylated carbon nanotubes, surface of which was coated...

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Bibliographic Details
Published in:Semiconductors (Woodbury, N.Y.) N.Y.), 2018-12, Vol.52 (14), p.1836-1838
Main Authors: Grushevskaya, H. V., Krylova, N. G., Lipnevich, I. V., Babenka, A. S., Egorova, V. P., Chakukov, R. F.
Format: Article
Language:English
Subjects:
ANIMAL TISSUES
Bundles
Carbon
CARBON NANOTUBES
Deoxyribonucleic acid
DIPOLES
DNA
ELECTROCHEMISTRY
ELECTRODES
ELECTRONS
FERMI LEVEL
Genetic testing
HIGH SPIN STATES
LAYERS
Magnetic Materials
Magnetism
METALS
NANOSCIENCE AND NANOTECHNOLOGY
Nanostructure Devices
NEOPLASMS
Nucleic acids
NUCLEOTIDES
ONCOGENES
OSCILLATIONS
Physics
Physics and Astronomy
PLASMONS
Polymorphism
PROBES
Selectivity
SENSITIVITY
SENSORS
SIGNALS
STABILIZATION
SURFACE COATING
SURFACES
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Summary:A label-free high-performance electrochemical sensing of single nucleotide polymorphism in native genomic DNA samples from cancer tissues, in oncogene-specific complementary DNA and in amplicons has been carried out on bundles of few-walled carboxylated carbon nanotubes, surface of which was coated with electron-dense layer of probe DNA. High selectivity and sensitivity of the novel DNA-nanosensor are ensured by the following features. Firstly, a complementary-hybridization signal is enhanced by resonance between plasmons in carbon nanotubes and dipole oscillations of hydrated nucleic acids located in near-electrode Helmholtz double layer of the impedance sensor. Secondly, a decoration of carbon nanotubes by adhered metal atoms in a high-spin state leads to Fermi level stabilization for the carbon nanotube bundles.
ISSN:1063-7826
1090-6479
DOI:10.1134/S1063782618140117