Analytical Approach for Detection of Ergosterol in Mushrooms Based on Modification Free Electrochemical Sensor in Organic Solvents

Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitr...

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Bibliographic Details
Published in:Food analytical methods 2018-09, Vol.11 (9), p.2590-2596
Main Authors: Vukojević, Vesna, Djurdjić, Sladjana, Švorc, Ľubomír, Veličković, Tanja Ćirković, Mutić, Jelena, Stanković, Dalibor M.
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Acetonitrile
AMMONIUM COMPOUNDS
Analytical Chemistry
Boron
Chemical sensors
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
DETECTION
Diamonds
Dichloromethane
DOPED MATERIALS
ELECTROCHEMISTRY
ELECTRODES
ERGOSTEROL
Food Science
Fungi
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
Mathematical analysis
Membranes
METHYLENE CHLORIDE
Microbiology
MUSHROOMS
ORGANIC SOLVENTS
Oxidation
PHOSPHATES
Plasma membranes
SILVER
Solvents
Spectrophotometry
Square waves
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Summary:Ergosterol (Erg) is the major sterol component in the plasma membranes of fungi. The main goal of this work was to develop rapid, reliable, and cost-effective method toward Erg detection based on application of unmodified boron-doped diamond electrode. Several organic solvents were tested (acetonitrile, dimethyformamide, dimethylsulfoxide, dichloromethane), and it was found that best analytical characteristics were achieved using acetonitrile containing 0.1 M tetrabutylammonium hexa-fluorophosphate as supporting electrolyte. Oval shaped and well-defined oxidation peak was recorded at potential of around 1.17 V versus Ag/Ag + non-aqueous reference electrode. Under optimized experimental conditions, linear working range from 2 to 200 μM was estimated, with limit of detection of 0.7 μM, using square wave voltammetry. Negligible effect of possible interfering compound was observed. Proposed methodology was successfully applied for estimation of Erg content in mushrooms extract with satisfactory results in comparison with spectrophotometric procedure. Obtained results clearly show that developed analytical methodology can be adequate replacement for the, up to date, used methods and open-novel approach for ergosterol detection.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-018-1249-3