An Eco-friendly Method of Purification for Xylanase from Aspergillus niger by Polyelectrolyte Precipitation

The separation of xylanase (Xyl) from a fungal extract obtained by submerged fermentation (SmF) was carried out using precipitation with natural polyelectrolytes ɩ-carrageenan (Carr) and chitosan (CHS). Xyl activity determined from the fungal extract at different times was modeled by mixed linear mo...

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Bibliographic Details
Published in:Journal of polymers and the environment 2019-12, Vol.27 (12), p.2895-2905
Main Authors: Podestá, María Victoria, Morilla, Esteban Amador, Allasia, María Belén, Woitovich Valetti, Nadia, Tubio, Gisela, Boggione, María Julia
Format: Article
Language:English
Subjects:
AMINO ACIDS
ASPERGILLUS
Carrageenan
Chemical precipitation
Chemistry
Chemistry and Materials Science
Chitosan
ELECTROLYTES
ELECTROPHORESIS
Environmental Chemistry
Environmental Engineering/Biotechnology
FERMENTATION
Fungi
Industrial Chemistry/Chemical Engineering
Macromolecules
Mass spectrometry
MASS SPECTROSCOPY
MATERIALS SCIENCE
Molecular weight
OLIGOSACCHARIDES
Original Paper
pH effects
PH VALUE
Polyelectrolytes
Polymer Sciences
Polymers
PRECIPITATION
Proteins
PURIFICATION
Reagents
REGRESSION ANALYSIS
Regression models
SPLINE FUNCTIONS
XYLANASE
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Summary:The separation of xylanase (Xyl) from a fungal extract obtained by submerged fermentation (SmF) was carried out using precipitation with natural polyelectrolytes ɩ-carrageenan (Carr) and chitosan (CHS). Xyl activity determined from the fungal extract at different times was modeled by mixed linear model for longitudinal data considering the factors as fixed effects and the experimental units as random effects. A spline regression was used to model the Xyl activity with knots at days 2 and 3 and interaction between three factors (p 
ISSN:1566-2543
1572-8919
DOI:10.1007/s10924-019-01571-3