miR-190 promotes HCC proliferation and metastasis by targeting PHLPP1

miRNAs regulate gene expression and enable clinicians to distinguish between benign and malignant tissues in cancers. PH domain leucine-rich repeat-containing protein phosphatase 1 (PHLPP1) is known to be a tumour suppressor. A lentiviral overexpression system was used to stably express miR-190, lea...

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Bibliographic Details
Published in:Experimental cell research 2018-10, Vol.371 (1), p.185-195
Main Authors: Xiong, Yuzhen, Wu, Shang, Yu, Huajun, Wu, Jun, Wang, Yajun, Li, Huimin, Huang, Hui, Zhang, Haitao
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
DIAGNOSIS
Hepatocellular carcinoma
HEPATOMAS
LEUCINE
LUNGS
METASTASES
Metastasis
MiR-190
PHLPP1
PHOSPHATASES
PHOSPHORYLATION
Proliferation
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Summary:miRNAs regulate gene expression and enable clinicians to distinguish between benign and malignant tissues in cancers. PH domain leucine-rich repeat-containing protein phosphatase 1 (PHLPP1) is known to be a tumour suppressor. A lentiviral overexpression system was used to stably express miR-190, leading to the enhancement of hepatocellular carcinoma (HCC) proliferation and metastasis as a result of inhibited PHLPP1 expression. The results showed that stable miR-190 expression increased the expression of EMT-related proteins (Snail and TCF8/ZEB1) as well as the phosphorylation of Akt at Ser473 and the expression of a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1). However, restoring PHLPP1 expression counteracted the effects of miR-190 on HCC proliferation, migration and invasion. The results of the animal experiments showed that miR-190 improved the HepG2 cell tumour formation and lung metastasis ability. Stable miR-190 overexpression leads to the downregulation of PHLPP1 protein expression. miR-190 has potential as a target in the treatment and diagnosis of HCC. [Display omitted] •miR-190 enhances the proliferation and metastasis of HCC.•miR-190 specifically inhibits PHLPP1 expression.•miR-190 may serve as a potential target and marker for HCC treatment.
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2018.08.008