miR-125b-5p inhibits breast cancer cell proliferation, migration and invasion by targeting KIAA1522

• Published in: Biochemical and biophysical research communications 2018-09, Vol.504 (1), p.277-282
• Main Authors: Li, Yongzhen, Wang, Yongxia, Fan, Hongzhe, Zhang, Zheying, Li, Na
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; Breast cancer; CELL PROLIFERATION; COLONY FORMATION; KIAA1522; LUCIFERASE; MAMMARY GLANDS; miR-125b-5p; NEOPLASMS; ONCOGENES; Tumor suppressor; WOUNDS
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.08.172

Abnormal gene expression due to the dysregulation of microRNAs (miRNAs) often occurred in the initiation or progression of cancers. The aim of this present study was to investigate the function role of miR-125b-5p in breast cancer (BC). Expression levels of miR-125b-5p were determined by quantitative Real-time PCR. Biological functions of miR-125b-5p in the progression of BC were investigated with a series of in vitro experiments including cell counting kit-8 assay, colony formation assay, wound-healing assay and transwell invasion assay. The target of miR-125b-5p in BC was validated by luciferase activity reporter assay and western blot assay. We found miR-125b-5p expression was significantly reduced in BC cell lines compared to the normal breast epithelial cell line. Functional assays showed that cell proliferation, colony formation ability, cell migration, and cell invasion can be suppressed by miR-125b-5p overexpression. Besides, KIAA1522 was validated as a direct target of miR-125b-5p in BC. Collectively, our study showed that miR-125b-5p functions as a tumor suppressor and regulates BC progression through targeting KIAA1522. •miR-125b-5p expression was found downregulated in breast cancer.•We explored the tumor suppressive role of miR-125b-5p in breast cancer.•KIAA1522 was reported to function as oncogene in breast cancer.•KIAA1522 was a direct target of miR-125b-5p in breast cancer.