Loading…
Effects of amiloride on physiological activity of stem cells of human lung cancer and possible mechanism
Lung cancer is a common malignant tumor, the cancer stem cells (CSCs) were regarded responsible for the development of cancer tissue. The effects of amiloride on lung cancer stem cells and the possible mechanism were not much investigated. In this study, human NCI-H1975 lung CSCs were selected by fl...
Saved in:
Published in: | Biochemical and biophysical research communications 2018-09, Vol.504 (1), p.1-5 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133 |
---|---|
cites | cdi_FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133 |
container_end_page | 5 |
container_issue | 1 |
container_start_page | 1 |
container_title | Biochemical and biophysical research communications |
container_volume | 504 |
creator | Zhang, Hengshuo Peng, Caixia Huang, He Lai, Yongxin Hu, Chenchen Li, Fei Wang, Degui |
description | Lung cancer is a common malignant tumor, the cancer stem cells (CSCs) were regarded responsible for the development of cancer tissue. The effects of amiloride on lung cancer stem cells and the possible mechanism were not much investigated. In this study, human NCI-H1975 lung CSCs were selected by flow cytometry, and the effects of amiloride at different concentrations (0, 12.5, 25, 50, and 100 μmol/L) were evaluated on proliferation, migration, invasion and apoptosis of CSCs using cell counting kit-8 and Transwell migration assays as well as flow cytometry. Wstern blot analysis was performed to investigate the effect of amiloride on the level of proteins in uPA system, NF-kB pathway, and PI3K-AKT-mTOR pathway in CSCs. As a result, we found that amiloride inhibited proliferation, migration and invasion of lung CSCs, and promoted apoptosis. Further, we found that amiloride decreased levels of target proteins in the uPA system, as well as the NF-kB and PI3K-AKT-mTOR pathways. These results indicated that amiloride could inhibit proliferation, migration and invasion of lung CSCs, and promotes apoptosis, these effects may be related to decreased levels of proteins in the uPA system, the NF-kB pathway, and the PI3K-AKT-mTOR pathway. |
doi_str_mv | 10.1016/j.bbrc.2018.06.138 |
format | article |
fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_23103545</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X18314517</els_id><sourcerecordid>2062835734</sourcerecordid><originalsourceid>FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133</originalsourceid><addsrcrecordid>eNp9kU1v1DAYhC0EokvhD3BAlrhwSXj97UhcUNUWpEpcqNSb5ThO16vEXuyk0v57HLZwRD748sxo5h2E3hNoCRD5-dD2fXYtBaJbkC1h-gXaEeigoQT4S7QDANnQjjxcoDelHAAI4bJ7jS5o1wmtNd-h_fU4ercUnEZs5zClHAaPU8TH_amENKXH4OyErVvCU1hOG1YWP2Pnp-mPaL_ONuJpjY_Y2eh8xjYO-JhKCf3k8ezd3sZQ5rfo1Win4t89_5fo_ub659W35u7H7ferr3eNY5ovjVBSMMkVZb1jHRFKOz5w4QfZaUWYAtVbJTTlvLO9oroH5UYnBstkfYSxS_Tx7JvKEkxxYakJXIqxtjSUEWCCi0p9OlPHnH6tvixmDmXrZKNPazEUJNVMKMYrSs-oy7VU9qM55jDbfDIEzLaDOZhtB7PtYECaukMVfXj2X_vZD_8kfw9fgS9nwNdbPAWft6i-3m8IeUs6pPA__98gTpg9</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2062835734</pqid></control><display><type>article</type><title>Effects of amiloride on physiological activity of stem cells of human lung cancer and possible mechanism</title><source>ScienceDirect Journals</source><creator>Zhang, Hengshuo ; Peng, Caixia ; Huang, He ; Lai, Yongxin ; Hu, Chenchen ; Li, Fei ; Wang, Degui</creator><creatorcontrib>Zhang, Hengshuo ; Peng, Caixia ; Huang, He ; Lai, Yongxin ; Hu, Chenchen ; Li, Fei ; Wang, Degui</creatorcontrib><description>Lung cancer is a common malignant tumor, the cancer stem cells (CSCs) were regarded responsible for the development of cancer tissue. The effects of amiloride on lung cancer stem cells and the possible mechanism were not much investigated. In this study, human NCI-H1975 lung CSCs were selected by flow cytometry, and the effects of amiloride at different concentrations (0, 12.5, 25, 50, and 100 μmol/L) were evaluated on proliferation, migration, invasion and apoptosis of CSCs using cell counting kit-8 and Transwell migration assays as well as flow cytometry. Wstern blot analysis was performed to investigate the effect of amiloride on the level of proteins in uPA system, NF-kB pathway, and PI3K-AKT-mTOR pathway in CSCs. As a result, we found that amiloride inhibited proliferation, migration and invasion of lung CSCs, and promoted apoptosis. Further, we found that amiloride decreased levels of target proteins in the uPA system, as well as the NF-kB and PI3K-AKT-mTOR pathways. These results indicated that amiloride could inhibit proliferation, migration and invasion of lung CSCs, and promotes apoptosis, these effects may be related to decreased levels of proteins in the uPA system, the NF-kB pathway, and the PI3K-AKT-mTOR pathway.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2018.06.138</identifier><identifier>PMID: 29958884</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>60 APPLIED LIFE SCIENCES ; Amiloride ; APOPTOSIS ; Lung cancer ; LUNGS ; NEOPLASMS ; PROTEINS ; Stem cell ; STEM CELLS</subject><ispartof>Biochemical and biophysical research communications, 2018-09, Vol.504 (1), p.1-5</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133</citedby><cites>FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29958884$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/23103545$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hengshuo</creatorcontrib><creatorcontrib>Peng, Caixia</creatorcontrib><creatorcontrib>Huang, He</creatorcontrib><creatorcontrib>Lai, Yongxin</creatorcontrib><creatorcontrib>Hu, Chenchen</creatorcontrib><creatorcontrib>Li, Fei</creatorcontrib><creatorcontrib>Wang, Degui</creatorcontrib><title>Effects of amiloride on physiological activity of stem cells of human lung cancer and possible mechanism</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Lung cancer is a common malignant tumor, the cancer stem cells (CSCs) were regarded responsible for the development of cancer tissue. The effects of amiloride on lung cancer stem cells and the possible mechanism were not much investigated. In this study, human NCI-H1975 lung CSCs were selected by flow cytometry, and the effects of amiloride at different concentrations (0, 12.5, 25, 50, and 100 μmol/L) were evaluated on proliferation, migration, invasion and apoptosis of CSCs using cell counting kit-8 and Transwell migration assays as well as flow cytometry. Wstern blot analysis was performed to investigate the effect of amiloride on the level of proteins in uPA system, NF-kB pathway, and PI3K-AKT-mTOR pathway in CSCs. As a result, we found that amiloride inhibited proliferation, migration and invasion of lung CSCs, and promoted apoptosis. Further, we found that amiloride decreased levels of target proteins in the uPA system, as well as the NF-kB and PI3K-AKT-mTOR pathways. These results indicated that amiloride could inhibit proliferation, migration and invasion of lung CSCs, and promotes apoptosis, these effects may be related to decreased levels of proteins in the uPA system, the NF-kB pathway, and the PI3K-AKT-mTOR pathway.</description><subject>60 APPLIED LIFE SCIENCES</subject><subject>Amiloride</subject><subject>APOPTOSIS</subject><subject>Lung cancer</subject><subject>LUNGS</subject><subject>NEOPLASMS</subject><subject>PROTEINS</subject><subject>Stem cell</subject><subject>STEM CELLS</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kU1v1DAYhC0EokvhD3BAlrhwSXj97UhcUNUWpEpcqNSb5ThO16vEXuyk0v57HLZwRD748sxo5h2E3hNoCRD5-dD2fXYtBaJbkC1h-gXaEeigoQT4S7QDANnQjjxcoDelHAAI4bJ7jS5o1wmtNd-h_fU4ercUnEZs5zClHAaPU8TH_amENKXH4OyErVvCU1hOG1YWP2Pnp-mPaL_ONuJpjY_Y2eh8xjYO-JhKCf3k8ezd3sZQ5rfo1Win4t89_5fo_ub659W35u7H7ferr3eNY5ovjVBSMMkVZb1jHRFKOz5w4QfZaUWYAtVbJTTlvLO9oroH5UYnBstkfYSxS_Tx7JvKEkxxYakJXIqxtjSUEWCCi0p9OlPHnH6tvixmDmXrZKNPazEUJNVMKMYrSs-oy7VU9qM55jDbfDIEzLaDOZhtB7PtYECaukMVfXj2X_vZD_8kfw9fgS9nwNdbPAWft6i-3m8IeUs6pPA__98gTpg9</recordid><startdate>20180926</startdate><enddate>20180926</enddate><creator>Zhang, Hengshuo</creator><creator>Peng, Caixia</creator><creator>Huang, He</creator><creator>Lai, Yongxin</creator><creator>Hu, Chenchen</creator><creator>Li, Fei</creator><creator>Wang, Degui</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20180926</creationdate><title>Effects of amiloride on physiological activity of stem cells of human lung cancer and possible mechanism</title><author>Zhang, Hengshuo ; Peng, Caixia ; Huang, He ; Lai, Yongxin ; Hu, Chenchen ; Li, Fei ; Wang, Degui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>60 APPLIED LIFE SCIENCES</topic><topic>Amiloride</topic><topic>APOPTOSIS</topic><topic>Lung cancer</topic><topic>LUNGS</topic><topic>NEOPLASMS</topic><topic>PROTEINS</topic><topic>Stem cell</topic><topic>STEM CELLS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hengshuo</creatorcontrib><creatorcontrib>Peng, Caixia</creatorcontrib><creatorcontrib>Huang, He</creatorcontrib><creatorcontrib>Lai, Yongxin</creatorcontrib><creatorcontrib>Hu, Chenchen</creatorcontrib><creatorcontrib>Li, Fei</creatorcontrib><creatorcontrib>Wang, Degui</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hengshuo</au><au>Peng, Caixia</au><au>Huang, He</au><au>Lai, Yongxin</au><au>Hu, Chenchen</au><au>Li, Fei</au><au>Wang, Degui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of amiloride on physiological activity of stem cells of human lung cancer and possible mechanism</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2018-09-26</date><risdate>2018</risdate><volume>504</volume><issue>1</issue><spage>1</spage><epage>5</epage><pages>1-5</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Lung cancer is a common malignant tumor, the cancer stem cells (CSCs) were regarded responsible for the development of cancer tissue. The effects of amiloride on lung cancer stem cells and the possible mechanism were not much investigated. In this study, human NCI-H1975 lung CSCs were selected by flow cytometry, and the effects of amiloride at different concentrations (0, 12.5, 25, 50, and 100 μmol/L) were evaluated on proliferation, migration, invasion and apoptosis of CSCs using cell counting kit-8 and Transwell migration assays as well as flow cytometry. Wstern blot analysis was performed to investigate the effect of amiloride on the level of proteins in uPA system, NF-kB pathway, and PI3K-AKT-mTOR pathway in CSCs. As a result, we found that amiloride inhibited proliferation, migration and invasion of lung CSCs, and promoted apoptosis. Further, we found that amiloride decreased levels of target proteins in the uPA system, as well as the NF-kB and PI3K-AKT-mTOR pathways. These results indicated that amiloride could inhibit proliferation, migration and invasion of lung CSCs, and promotes apoptosis, these effects may be related to decreased levels of proteins in the uPA system, the NF-kB pathway, and the PI3K-AKT-mTOR pathway.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29958884</pmid><doi>10.1016/j.bbrc.2018.06.138</doi><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0006-291X |
ispartof | Biochemical and biophysical research communications, 2018-09, Vol.504 (1), p.1-5 |
issn | 0006-291X 1090-2104 |
language | eng |
recordid | cdi_osti_scitechconnect_23103545 |
source | ScienceDirect Journals |
subjects | 60 APPLIED LIFE SCIENCES Amiloride APOPTOSIS Lung cancer LUNGS NEOPLASMS PROTEINS Stem cell STEM CELLS |
title | Effects of amiloride on physiological activity of stem cells of human lung cancer and possible mechanism |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T04%3A16%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20amiloride%20on%20physiological%20activity%20of%20stem%20cells%20of%20human%20lung%20cancer%20and%20possible%20mechanism&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Zhang,%20Hengshuo&rft.date=2018-09-26&rft.volume=504&rft.issue=1&rft.spage=1&rft.epage=5&rft.pages=1-5&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2018.06.138&rft_dat=%3Cproquest_osti_%3E2062835734%3C/proquest_osti_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c384t-5765364723bc391578c4d45ed698713707ba7582449ab728b07cfc5da36363133%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2062835734&rft_id=info:pmid/29958884&rfr_iscdi=true |