Long non-coding RNA EPIC1 promotes human lung cancer cell growth

Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a MYC-interacting LncRNA. In the present study, the expression and potential function of Lnc-EPIC1 in human lung cancer cells are tested. We show that Lnc-EPIC1 expression is significantly higher in established/primary human lung cancer cells than th...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-09, Vol.503 (3), p.1342-1348
Main Authors: Zhang, Bing, Lu, Hui-Yu, Xia, Yun-Hong, Jiang, Ai-Gui, Lv, Yu-Xin
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
APOPTOSIS
CELL CYCLE
Cell Proliferation
EPIC1
Female
Humans
Long non-coding RNA (LncRNA)
Lung cancer
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
LUNGS
Middle Aged
MYC and cell growth
NEOPLASMS
Proto-Oncogene Proteins c-myc - metabolism
RNA
RNA, Long Noncoding - metabolism
Tumor Cells, Cultured
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Summary:Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a MYC-interacting LncRNA. In the present study, the expression and potential function of Lnc-EPIC1 in human lung cancer cells are tested. We show that Lnc-EPIC1 expression is significantly higher in established/primary human lung cancer cells than that in human lung epithelial cells. Lnc-EPIC1 is also elevated in human lung cancer tissues. Silencing of Lnc-EPIC1 by targeted siRNA significantly inhibited human lung cancer cell growth, survival and proliferation, whiling inducing G1-S cell cycle arrest and cell apoptosis. MYC targets, including Cyclin A1, CDC20 and CDC45, were downregulated by Lnc-EPIC1 siRNA. MYC knockout by CRISPR/Cas-9 gene-editing method mimicked actions by Lnc-EPIC1 siRNA in A549 cells. Conversely, forced overexpression of Lnc-EPIC1 by a lentiviral construct increased expression of MYC targets to promote A549 cell growth. Lnc-EPIC1 directly associated with MYC protein in the nuclei of A549 cells. Significantly MYC knockout abolished Lnc-EPIC1 silencing- or overexpression-induced actions in human lung cancer cells. Together, our results show that Lnc-EPIC1 promotes human lung cancer cell growth possibly by targeting MYC. •Lnc-EPIC1 expression is elevated in human lung cancer cells and tissues.•Lnc-EPIC1 siRNA inhibits lung cancer cell growth, but inducing cell cycle arrest and apoptosis.•MYC is the primary target of Lnc-EPIC1 in lung cancer cells.•Lnc-EPIC1 overexpression promotes lung cancer cell growth.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.07.046