Detection of mycobacterial small RNA in the bacterial culture supernatant and plasma of patients with active tuberculosis

Bacterial small RNA (sRNA) has been shown to play an important role in control of bacteria virulence, stress response and physiological metabolism by post-transcriptional regulation of gene expression. However, there were few reports about bacterial sRNA as a biomarker of infection. To test the pote...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-09, Vol.503 (2), p.490-494
Main Authors: Fu, Yingmei, Li, Wenjing, Wu, Zheng, Tao, Yuanmeihui, Wang, Xinyang, Wei, Jing, Jiang, Peidong, Wu, Jinyin, Zhang, Zhiwei, Zhang, Wenli, Zhao, Jizi, Zhang, Fengmin
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
Bacteriological Techniques
Base Sequence
BIOLOGICAL MARKERS
Biomarker
Cattle
Humans
Mycobacterium bovis - genetics
Mycobacterium bovis - isolation & purification
MYCOBACTERIUM TUBERCULOSIS
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Real-Time Polymerase Chain Reaction
RNA
RNA, Bacterial - analysis
RNA, Bacterial - blood
RNA, Bacterial - genetics
RNA, Small Untranslated - analysis
RNA, Small Untranslated - blood
RNA, Small Untranslated - genetics
Sequence Alignment
sRNA
TB patients
TUBERCULOSIS
Tuberculosis - blood
Tuberculosis - microbiology
Tuberculosis, Bovine - blood
Tuberculosis, Bovine - microbiology
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Summary:Bacterial small RNA (sRNA) has been shown to play an important role in control of bacteria virulence, stress response and physiological metabolism by post-transcriptional regulation of gene expression. However, there were few reports about bacterial sRNA as a biomarker of infection. To test the potential role of sRNA in indicating infection of Mycobacterium tuberculosis, total RNA were extracted from the filtrated bacterial cultural supernatant. After synthesis of cDNA by reverse transcription, four Mycobacterial sRNAs including ASdes, ASpks, AS1726, and AS1890, which have been experimentally confirmed by Kristine B in the year of 2009, were detected by real time PCR. The specificity was verified by sequencing of the amplified products. Moreover, we demonstrate that the presence of sRNA Asdes in plasma of 55.56% (15/27) TB patients and 25.00% (6/24) normal controls with BCG vaccination (P 
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.04.165