Efficient mRNA delivery system utilizing chimeric VSVG-L7Ae virus-like particles

The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types, and thus new delivery methods are needed. To this...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-11, Vol.505 (4), p.1097-1102
Main Authors: Zhitnyuk, Yulia, Gee, Peter, Lung, Mandy S.Y., Sasakawa, Noriko, Xu, Huaigeng, Saito, Hirohide, Hotta, Akitsu
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
DNA
Gene Transfer Techniques
HEK293 Cells
Humans
L7Ae RNA-binding protein
Membrane Glycoproteins - chemistry
MESSENGER-RNA
MONOCYTES
mRNA delivery
Ribosomal Proteins - chemistry
RNA, Messenger - genetics
STEM CELLS
Viral Envelope Proteins - chemistry
VLPs
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Summary:The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types, and thus new delivery methods are needed. To this end, we have established a novel mRNA delivery system utilizing chimeric virus-like particles (VLPs). We generated a novel VLP by fusing protein G of Vesicular stomatitis virus (VSV-G) with a ribosomal protein L7Ae of Archeoglobus fulgidus. This system allowed the efficient delivery of EGFP mRNA which was independent from the presence of BoxC/D motif in the mRNA sequence. Our VSVG-L7Ae VLP system demonstrated high transduction efficacy in hard-to-transfect cell lines, such as human induced pluripotent stem cells (iPS cells) and monocytes. In summary, this platform may serve as an efficient and transient transgene delivery tool for an mRNA of interest. •Generation of chimeric virus-like particles by fusion of VSV-G and L7Ae proteins.•VSVG-L7Ae VLPs efficiently incorporate and deliver mRNA into various human cell lines.•Inclusion of mRNA is mediated by RNA binding domain of L7Ae.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.09.113