Suppression of Elp2 prevents renal fibrosis and inflammation induced by unilateral ureter obstruction (UUO) via inactivating Stat3-regulated TGF-β1 and NF-κB pathways

• Published in: Biochemical and biophysical research communications 2018-06, Vol.501 (2), p.400-407
• Main Authors: Lu, Shan, Fan, Hong-Wu, Li, Kai, Fan, Xiao-Di
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; Elp2; INFLAMMATION; KIDNEYS; LYMPHOKINES; MICE; Renal fibrosis and inflammation; RNA POLYMERASES; STAT3; TGF-β1; UUO
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.04.227

Renal fibrosis and inflammation are common underlying processes of progressive kidney diseases. Elongator protein 2 (Elp2), identical to signal transducer and activator of transcription-3 (STAT-3)-interacting protein-1 (Stip1), is a component of the Elongator complex that regulates RNA polymerase II. Elp2 regulates STAT-3 activation to control various cellular processes. The mechanisms of Elp2 prevention in renal interstitial fibrosis and inflammation remain unknown. In the study, Elp2 transgenic knockout (KO) and wild type (WT) mice were employed to investigate the effects of Elp2 on renal fibrosis and inflammation development after unilateral ureter obstruction (UUO) surgery. The results indicted that Elp2 was significantly expressed in renal tissues of WT/UUO mice. Elp2-KO mice exhibited attenuated histological changes of kidney, as well as collagen and fibrosis accumulation. Lower expressions of transforming growth factor (TGF)-β1, α-smooth muscle actin (α-SMA), fibronectin, vimentin, and phospho-Smad2/3 were observed in kidney of Elp2-KO mice than that of WT mice after UUO. Elp2-KO mice showed less inflammation, as evidenced by the decrease of circulating or renal pro-inflammatory cytokines, as well as the reduction of phospho-nuclear factor (NF)-κB. Additionally, Elp2-KO apparently led to a decrease in phospho-STAT3 in kidney of UUO mice. In vitro, we found that TGF-β1- and LPS-induced fibrosis and inflammation were abrogated by Elp2 knockdown, which were intriguingly abolished by activating STAT3 phosphorylation using its activator of colivelin (Col). Together, our findings supplied that Elp2 might be a potential therapeutic target to prevent the progression of renal fibrosis and inflammation. •Elp2 knockout attenuates fibrotic progression in a UUO mouse model.•Elp2 deficiency reduces tubulointerstitial inflammation in the UUO model.•Elp2 knockdown ameliorates TGF-β1-induced fibrosis and LPS-triggered inflammation in mTECs through regulating STAT3.