Low-intensity pulsed ultrasound induces cartilage matrix synthesis and reduced MMP13 expression in chondrocytes

Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics. In previous in vivo and in vitro studies, LIPUS has been shown to have promising effects on cellular elements in articular cartilage, particularly chondrocytes in patients with osteoarthritis. However, the effects of LI...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-11, Vol.506 (1), p.290-297
Main Authors: Sekino, Jumpei, Nagao, Mayu, Kato, Shunichiro, Sakai, Mayu, Abe, Kimiko, Nakayama, Enri, Sato, Mitsuyasu, Nagashima, Yuki, Hino, Haruka, Tanabe, Natsuko, Kawato, Takayuki, Maeno, Masao, Suzuki, Naoto, Ueda, Koichiro
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Aggrecan
BONE FRACTURES
CARTILAGE
Collagen synthesis
HEALING
LIPUS
MMP13
SKELETON
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Summary:Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics. In previous in vivo and in vitro studies, LIPUS has been shown to have promising effects on cellular elements in articular cartilage, particularly chondrocytes in patients with osteoarthritis. However, the effects of LIPUS on the cellular mechanisms through which LIPUS alters extracellular matrix (ECM) synthesis in chondrocytes are unclear. In this study, we investigated the effects of the optimal intensity and cellular mechanisms of LIPUS on the regeneration of cartilage matrix in chondrocytes. LIPUS induced collagen synthesis and the remodeling of aggrecan via the activation of ERK1/2. In contrast, MMP13 expression was decreased in chondrocytes. Additionally, chondrocytes responded optimally to LIPUS at an intensity higher than the clinical setting for bone fracture healing. These results suggested that LIPUS induced ECM regeneration via increases in hypertrophic chondrocytes and delayed endochondral ossification in chondrocytes. •LIPUS induces collagen synthesis.•LIPUS suppresses MMP13 expression.•LIPUS induces aggrecan remodeling.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.10.063