Solasodine reverses stemness and epithelial-mesenchymal transition in human colorectal cancer

Adverse side effects of conventional chemotherapy, acquired resistance and fatal tumor metastasis of human colorectal cancer (CRC) are propelling the exploration for novel selective anticarcinogens. Solasodine is a main active component isolated from Solanum incanum L that exhibited a potent stemnes...

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Published in:Biochemical and biophysical research communications 2018-10, Vol.505 (2), p.485-491
Main Authors: Zhuang, Yu-wen, Wu, Cun-en, Zhou, Jin-yong, Zhao, Zhi-ming, Liu, Chun-li, Shen, Jun-yi, Cai, Hui, Liu, Shen-lin
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
CHEMOTHERAPY
Colorectal cancer
DRUGS
Epithelial-mesenchymal transition
HERBS
Migration
NEOPLASMS
SOLANUM
Solasodine
Stemness
WOUNDS
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Summary:Adverse side effects of conventional chemotherapy, acquired resistance and fatal tumor metastasis of human colorectal cancer (CRC) are propelling the exploration for novel selective anticarcinogens. Solasodine is a main active component isolated from Solanum incanum L that exhibited a potent stemness and invasion inhibitory effect on human colorectal cancer HCT116 cells. Colony Spheroid formation assay showed that solasodine dose-dependently prohibited HCT116 cell stemness. CD133, CD44, Nanog, Oct-4 and Sox-2 were inhibited by solasodine to reverse stemness and similar mechanism was stimulated in vivo. Transwell and scratch wound assays revealed that solasodine impeded HCT116 cell invasion and migration potential strengthened by TGF-β1. Moreover, solasodine attenuated TGF-β1-induced EMT and decreased MMPs while in vivo study showed the same trend. The results of this study implied that solasodine may be a novel therapeutic drug for CRC treatment. •A component from the Chinese herb solanaceous species has CRC resistance effect.•We explore the induction of TGF-β1 on EMT and cell stemness.•Solasodine shows apparent effect of stemness and EMT reversion in vitro and vivo.•The effect of solasodine on EMT stemness molecules is researched.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.09.094