Berberine derivative, Q8, stimulates osteogenic differentiation

Berberine has been implicated to be involved in maintaining bone health due to its anti-oxidative and osteogenic properties. However, low potency and low bioavailability limit the clinical development of the drug. To overcome these obstacles, we previously synthesized a compound, Q8, which is a stru...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-09, Vol.504 (1), p.340-345
Main Authors: Han, Younho, Kim, Myeong Ji, Lee, Kwang Youl
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
ALKALINE PHOSPHATASE
Berberine
BIOLOGICAL AVAILABILITY
CONNECTIVE TISSUE CELLS
Osteoblast differentiation
OSTEOPOROSIS
OXIDATION
PHOSPHORYLATION
PPARγ
SKELETON
TRANSCRIPTION FACTORS
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Summary:Berberine has been implicated to be involved in maintaining bone health due to its anti-oxidative and osteogenic properties. However, low potency and low bioavailability limit the clinical development of the drug. To overcome these obstacles, we previously synthesized a compound, Q8, which is a structural homolog of berberine. The present study examined the pharmacological functions of Q8 to evaluate its potential use in bone regeneration with respect to osteoblast differentiation. Here, we report that Q8 enhanced BMP4-induced alkaline phosphatase (ALP) activity and transcription from the ALP promoter. In addition, Q8 suppressed the expression and activity of PPARγ (a known negative regulator of osteogenesis due to its stimulatory effects on adipogenesis and its role as an adipogenic transcription factor), which in turn increases β-catenin expression in the nucleus, and ultimately promotes osteoblast differentiation. Meanwhile, Q8 reversed the inhibitory effects of the PPARγ agonist, rosiglitazone, on osteoblast differentiation. This study demonstrated that Q8 promotes osteoblast differentiation via inhibition of PPARγ and the enhancement of osteoblast function by Q8 may contribute to the prevention for osteoporosis. [Display omitted] •Q8 increases BMP4-induced osteoblast differentiation of C2C12 cells.•Q8 induced β-catenin nuclear accumulation by enhancing GSK3β phosphorylation via inactivation of PPARγ.•Q8 ameliorated the inhibitory effects of rosiglitazone during osteoblast differentiation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.08.192