Auriculasin from Flemingia philippinensis roots shows good therapeutic indexes on hyperactive behavior in zebrafish

• Published in: Biochemical and biophysical research communications 2018-09, Vol.503 (3), p.1254-1259
• Main Authors: Wang, Tuoyi, Liu, Yang, Liu, Huazhen, Li, Chunyan, Wang, Yan
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; ADHD; Animals; Attention Deficit Disorder with Hyperactivity - drug therapy; Attention Deficit Disorder with Hyperactivity - metabolism; BIOLOGICAL EFFECTS; Circadian clock; DOPAMINE; Fabaceae - chemistry; Hyperactive behavior; Isoflavones; Isoflavones - chemistry; Isoflavones - isolation & purification; Isoflavones - pharmacology; Locomotion - drug effects; MELATONIN; per1b; Period Circadian Proteins - deficiency; Period Circadian Proteins - genetics; Period Circadian Proteins - metabolism; Plant Roots - chemistry; POLYMERASES; Zebrafish; Zebrafish Proteins - deficiency; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.07.033

Previously, period1b−/− zebrafish mutants were used to establish an attention deficit hyperactivity disorder (ADHD) model, in which hyperactive behavior was found to be a typical characteristic of ADHD due to down-regulated dopamine levels. Here, we used five prenylated isoflavones from Flemingia philippinensis roots to study their therapeutic effects on hyperactivity behavior in period1b−/− zebrafish. Results of locomotor activity assay showed that auriculasin, one of the prenylated isoflavones, significantly reduced the hyperactivity behavior in period1b−/− zebrafish. Hormone measurement results showed that auriculasin increased melatonin and dopamine content. Results of quantitative real-time polymerase chain reaction showed that auriculasin down-regulated the expression of mao but up-regulated the expression of th and per1b. Thus, auriculasin demonstrated a potential biological effect on dopamine activity to inhibit hyperactivity behavior in the ADHD zebrafish model by regulating circadian clock gene per1b. Drugs screening revealed that auriculasin modulated dopamine content and inhibited hyperactive behavior through circadian clock gene per1b in zebrafish. [Display omitted] •Auriculasin regulates dopamine secretion through per1b.•Auriculasin shows good therapeutic indexes on hyperactive behavior in zebrafish.•Auriculasin have potential therapeutic indexes on ADHD.