Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells

Bone remodeling is maintained through the balance between bone formation by osteoblasts and bone resorption by osteoclasts. Previous studies suggested that intracellular Ca2+ signaling plays an important role in the differentiation of osteoblasts; however, the molecular mechanism of Ca2+ signaling i...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-11, Vol.506 (3), p.716-722
Main Authors: Sato, Moe, Asano, Toshifumi, Hosomichi, Jun, Ono, Takashi, Nakata, Takao
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Animals
Calcium - metabolism
Calcium Channels - metabolism
CALCIUM IONS
Calcium Signaling
Cell Differentiation
Cell Line
CONNECTIVE TISSUE CELLS
Differentiation
Intracellular Ca2+ signaling
Intracellular Space - metabolism
Ion Channel Gating - radiation effects
Light
Mice, Inbred C57BL
Optogenetics
Osteoblast
Osteoclasts - cytology
Osteoclasts - metabolism
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Summary:Bone remodeling is maintained through the balance between bone formation by osteoblasts and bone resorption by osteoclasts. Previous studies suggested that intracellular Ca2+ signaling plays an important role in the differentiation of osteoblasts; however, the molecular mechanism of Ca2+ signaling in the differentiation of osteoblasts remains unclear. To elucidate the effect of Ca2+ signaling in osteoblasts, we employed an optogenetic tool, blue light-activated Ca2+ channel switch (BACCS). BACCS was used to spatiotemporally control intracellular Ca2+ with blue light stimulation. MC3T3-E1 cells, which have been used as a model of differentiation from preosteoblast to osteoblast, were promoted to differentiate by BACCS expression and rhythmical blue light stimulation. The results indicated that intracellular Ca2+ change from the outside of the cells can regulate signaling for differentiation of MC3T3-E1 cells. Our findings provide evidence that Ca2+ could cause osteoblast differentiation. •Intracellular Ca2+ signaling changes before and after differentiation of osteoblasts.•An optogenetic tool can control intracellular Ca2+ signaling of osteoblasts.•Using the optogenetic tool, differentiation of osteoblasts was enhanced.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.10.107