Co-transplantation of exosomes derived from hypoxia-preconditioned adipose mesenchymal stem cells promotes neovascularization and graft survival in fat grafting

Adipose-derived stromal cells (ADSCs)-derived exosomes (ADSC-Exos) account for the proangiogenic potential of stem cell. This study aimed to investigate the effect of ADSC-derived exosomes (ADSC-Exos) on the survival in fat grafting. A nude mouse model of subcutaneous fat grafting was adopted. Hypox...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2018-02, Vol.497 (1), p.305-312
Main Authors: Han, Yu-di, Bai, Yun, Yan, Xin-long, Ren, Jing, Zeng, Quan, Li, Xiao-dong, Pei, Xue-tao, Han, Yan
Format: Article
Language:English
Subjects:
60 APPLIED LIFE SCIENCES
Adipose-derived stem cells
ANGIOGENESIS
ANOXIA
Exosomes
Fat grafting
FATS
INFLAMMATION
INJECTION
Microenvironment
STEM CELLS
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Summary:Adipose-derived stromal cells (ADSCs)-derived exosomes (ADSC-Exos) account for the proangiogenic potential of stem cell. This study aimed to investigate the effect of ADSC-derived exosomes (ADSC-Exos) on the survival in fat grafting. A nude mouse model of subcutaneous fat grafting was adopted. Hypoxic preconditioned ADSC-Exos and ADSC-Exos were injected around the grafted tissue. The fat graft sample was weighed and examined by hematoxylin and eosin (H&E) staining and immunohistochemistry. Laser Doppler flowmetry and CD31 immunofluorescence staining were used to analyze neovascularization. ADSC-Exo and hypoxic ADSC-Exo groups had a significantly higher weight of fat graft and more perilipin-positive adipocytes than the control groups from 2 to 8 weeks after grafting, and the hypoxic ADSC-Exo group had better outcomes (all P 
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2018.02.076