Radiosensitization of a Mammalian Cell Line with 5-Bromodeoxyuridine

BS>Some radiobiological properties of a mammalian cell line after exposure to the halogenated nucleoside 5-bromodeoxyuridine (BUDR) are described. Incorporation of BUDR into the deoxyribonucleic acid of mammalian cells in culture to the extent of 1% was measured after 3 to 4 days of in cubation w...

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Bibliographic Details
Published in:Radiation research 1962-10, Vol.17 (4), p.479-491
Main Authors: Delihas, N., Rich, M. A., Eidinoff, M. L.
Format: Article
Language:English
Subjects:
ABSORPTION
ANIMAL CELLS
BIOCHEMISTRY
BROMINE COMPOUNDS
Bromodeoxyuridine
Cell culture techniques
Cell growth
Cell Line
Cell lines
DIAGRAMS
DNA
Giant cells
HeLa cells
IRRADIATION
MEASURED VALUES
Neutrons
NUCLEIC ACIDS
Nucleosides
Pyrimidines
Radiation Effects
RADIOBIOLOGY
RADIOLOGY AND NUCLEAR MEDICINE
RADIOSENSITIVITY
Research Design
THERMAL NEUTRONS
Tissue Culture Techniques
X RADIATION
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Summary:BS>Some radiobiological properties of a mammalian cell line after exposure to the halogenated nucleoside 5-bromodeoxyuridine (BUDR) are described. Incorporation of BUDR into the deoxyribonucleic acid of mammalian cells in culture to the extent of 1% was measured after 3 to 4 days of in cubation with 6 mu g of the analog per milliliter. An in creased x-ray sensitivity of about 2 and an increase in extrapolation number of survival curves were observed after exposure to the above-mentioned concentration of BUDR. In contrast to this, an enhanced sensitivity slightly greater than 1 and a decrease in extrapolation number were found after thermal neutron irradiation and identical treatment with BUDR. Theoretical calculations on x-ray energy absorbed by BUDR-exposed cells show that a negligible increase in energy is absorbed per cell with incorporation of 1% BUDR, as compared to unexposed cells. For analogexposed cells, survival was found to increase when a single dose of x rays was administered 4 to 12 hours after cell plating. For unexposed cells, survival was found to be constant during that same period.
ISSN:0033-7587
1938-5404
DOI:10.2307/3571174