Autoradiography Using Melted Nuclear Emulsion

During an investigation of rabbit uterus (labeled with C14) by the method of staining first, coating with nitrocellulose and emulsion, and then processing, it was found that sections became de-stained in spite of considerable overstaining, and black deposits, ranging from a few silver grains to larg...

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Bibliographic Details
Published in:Nature (London) 1961-11, Vol.192 (4801), p.437-439
Main Author: KULANGARA, A. C
Format: Article
Language:English
Subjects:
Autoradiography
CARBON 14
Emulsions
Humanities and Social Sciences
multidisciplinary
NUCLEAR EMULSIONS
Old Medline
RABBITS
RADIOAUTOGRAPHY
RADIOLOGY AND NUCLEAR MEDICINE
Science
Science (multidisciplinary)
TISSUES
TRACER TECHNIQUES
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Summary:During an investigation of rabbit uterus (labeled with C14) by the method of staining first, coating with nitrocellulose and emulsion, and then processing, it was found that sections became de-stained in spite of considerable overstaining, and black deposits, ranging from a few silver grains to large aggregates, appeared on slides. Discrete grains, indistinguishable from those caused by ionizing events, occurred in greater density near the aggregates. Observations are reported which indicate that stains may' be involved in the formation of artefacts when a thin nitrocellulose film is used and that artefacts as well as bleaching of stains may be prevented by embedding sections in a hydrophobic layer. Discussion is given on both strain and chemically induced artefacts, and also on protective coatings.
ISSN:0028-0836
1476-4687
DOI:10.1038/192437a0