Natural-abundance sup 13 C NMR study of glycogen repletion in human liver and muscle

Optimizing the surface-coil design and spectral-acquisition parameters has led to the observation of the {sup 13}C NMR natural abundance glycogen signal in man at 2.1 T. Both the human muscle and hepatic glycogen signals can be detected definitively with a time resolution of {approx}13 min. A {sup 1...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-03, Vol.86:5
Main Authors: Jue, T., Rothman, D.L., Tavitian, B.A., Shulman, R.G.
Format: Article
Language:English
Subjects:
550601 - Medicine- Unsealed Radionuclides in Diagnostics
BARYONS
BODY
CARBOHYDRATES
CARBON 13
CARBON ISOTOPES
DIGESTIVE SYSTEM
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
GLANDS
GLYCOGEN
HADRONS
ISOTOPES
LIGHT NUCLEI
LIVER
MAGNETIC RESONANCE
METABOLISM
MUSCLES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTONS
RADIOLOGY AND NUCLEAR MEDICINE
RESONANCE
SACCHARIDES
SPECTRA
STABLE ISOTOPES
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Summary:Optimizing the surface-coil design and spectral-acquisition parameters has led to the observation of the {sup 13}C NMR natural abundance glycogen signal in man at 2.1 T. Both the human muscle and hepatic glycogen signals can be detected definitively with a time resolution of {approx}13 min. A {sup 1}H/{sup 13}C concentric surface coil was used. The {sup 1}H outer coil was 11 cm in diameter; the {sup 13}C inner coil was 8 cm in diameter. The coils were tuned to 89.3 MHz and 22.4 MHz, respectively. The {sup 1}H coil was used for optimizing field homogeneity (shimming) the magnet and for single-frequency decoupling of the C{sub 1} glycogen signal. Total power deposition from both the transmitter pulse and the continuous wave decoupling did not exceed the Food and Drug Administration guideline of 8 W/kg of tissue. Experiments were done for which healthy subjects returned to the magnets at different times for {sup 13}C NMR measurement. The spectral difference between experiments was within the noise in the C{sub 1} glycogen region. Because of the spectral reproducibility and the signal sensitivity, hepatic glycogen repletion can be followed. Four hours postprandial, hepatic glycogen increases by 3.8 times from the basal fasted state. The hepatic glycogen data correspond directly to previous biopsy results and support the use of {sup 13}C NMR as a noninvasive probe of human metabolism.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.5.1439