Competitive Protein Binding Assay for Piritrexim

A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobac...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1989-09, Vol.78 (9), p.749-752
Main Authors: Woolley, Joseph L., Ringstad, Jennifer L., Sigel, Carl W.
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
Analysis
Antineoplastic Agents - metabolism
ANTINEOPLASTIC DRUGS
BACTERIA
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
Binding, Competitive
BIOCHEMICAL REACTION KINETICS
Biological and medical sciences
CHROMATOGRAPHY
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
Folic Acid Antagonists
General pharmacology
Humans
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
Iodine Radioisotopes
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
Medical sciences
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
OXIDOREDUCTASES
Pharmacology. Drug treatments
Protein Binding
Pyrimidines - metabolism
RADIOASSAY
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
TRACER TECHNIQUES
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Description
Summary:A competitive protein binding assay for piritrexim (PTX, 1) that makes use of a commercially available radioassay kit for methotrexate has been developed. After it is selectively extracted from plasma, PTX competes with [125l]methotrexate for binding to dihydrofolate reductase isolated from Lactobacillus casei. Free drug is separated from bound drug by adsorption to dextran-coated charcoal. Piritrexim is measurable over a range of 0.01 to 10.0, μg/mL in plasma with a coefficient of variation
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600780910