Allosteric and catalytic binding of S-adenosylmethionine to Escherichia coli DNA adenine methyltransferase monitored by 3H NMR

Adenine methylation of GATC sequences in DNA is carried out by the DNA adenine methyltransferase with the methyl group source being the cofactor S-adenosylmethionine. We report 3H NMR studies on the interaction of DNA adenine methyltransferase with S-adenosylmethionine and the reaction when the tern...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-08, Vol.88 (15), p.6394-6397
Main Authors: Bergerat, A, Guschlbauer, W, Fazakerley, G V
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
ADENINES
Allosteric Site
AMINES
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
BACTERIA
BASIC BIOLOGICAL SCIENCES
Binding Sites
BIOCHEMICAL REACTION KINETICS
CARBON-GROUP TRANSFERASES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DNA
DNA SEQUENCING
DRUGS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
ESCHERICHIA COLI
Escherichia coli - enzymology
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
Magnetic Resonance Spectroscopy - methods
MEMBRANE PROTEINS
METHIONINE
METHYL TRANSFERASES
METHYLATION
MICROORGANISMS
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
OLIGONUCLEOTIDES
Oligonucleotides - metabolism
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
PURINES
REACTION KINETICS
RECEPTORS
RESONANCE
S-Adenosylmethionine - metabolism
Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
TRANSFERASES
Tritium
TRITIUM COMPOUNDS
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Description
Summary:Adenine methylation of GATC sequences in DNA is carried out by the DNA adenine methyltransferase with the methyl group source being the cofactor S-adenosylmethionine. We report 3H NMR studies on the interaction of DNA adenine methyltransferase with S-adenosylmethionine and the reaction when the ternary complex is formed with an oligonucleotide containing a GATC site. The methylation reaction was also studied in the presence of a competitive inhibitor and this showed two successive stages involved in the methylation and two sites of binding for S-adenosylmethionine.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.15.6394