NF-kB activation by ultraviolet light not dependent on a nuclear signal

Exposure of mammalian cells to radiation triggers the ultraviolet (UV) response, which includes activation of activator protein-1 (AP-1) and nuclear factor kappa B (NF-kB). This was postulated to occur by induction of a nuclear signaling cascade by damaged DNA. Recently, induction of AP-1 by UV was...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1993-09, Vol.261:5127
Main Authors: Devary, Y., Rosette, C., DiDonato, J.A., Karin, M.
Format: Article
Language:English
Subjects:
560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
CELL MEMBRANES
ELECTROMAGNETIC RADIATION
ENZYMES
INDUCTION
MEMBRANES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RADIATION EFFECTS
RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT
RADIATIONS
TRANSCRIPTION FACTORS
TRANSFERASES
ULTRAVIOLET RADIATION
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Summary:Exposure of mammalian cells to radiation triggers the ultraviolet (UV) response, which includes activation of activator protein-1 (AP-1) and nuclear factor kappa B (NF-kB). This was postulated to occur by induction of a nuclear signaling cascade by damaged DNA. Recently, induction of AP-1 by UV was shown to be mediated by a pathway involving Src tyrosine kinases and the Ha-Ras small guanosine triphosphate-binding protein, proteins located at the plasma membrane. It is demonstrated here that the same pathway mediates induction of NF-kB by UV. Because inactive NF-kB is stored in the cytosol, analysis of its activation directly tests the involvement of a nuclear-initiated signaling cascade. Enucleated cells are fully responsive to UV both in NF-kB induction and in activation of another key signaling event. Therefore, the UV response does not require a signal generated in the nucleus and is likely to be initiated at or near the plasma membrane.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8367725