Membrane fusion and inverted phases

• Published in: Biochemistry (Easton) 1989-05, Vol.28 (9), p.3692-3703
• Main Authors: Ellens, Harma, Siegel, David P, Alford, Dennis, Yeagle, Philip L, Boni, Lawrence, Lis, Leonard J, Quinn, Peter J, Bentz, Joe
• Format: Article
• Language: English
• Subjects: 550201 - Biochemistry- Tracer Techniques; BASIC BIOLOGICAL SCIENCES; Calorimetry, Differential Scanning; CELL CONSTITUENTS; CELL MEMBRANES; Chemical Phenomena; Chemistry; COHERENT SCATTERING; DIFFRACTION; ELECTRON MICROSCOPY; ESTERS; Freeze Fracturing; ISOTOPE APPLICATIONS; ISOTOPES; Kinetics; LIGHT NUCLEI; LIPIDS; LIPOSOMES; MAGNETIC RESONANCE; Magnetic Resonance Spectroscopy; MEMBRANES; MICROSCOPY; Microscopy, Electron; Models, Theoretical; Molecular Conformation; NUCLEAR MAGNETIC RESONANCE; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANOIDS; PHASE STUDIES; PHOSPHOLIPIDS; PHOSPHORUS 31; PHOSPHORUS ISOTOPES; RESONANCE; SCATTERING; Scattering, Radiation; STABLE ISOTOPES; Structure-Activity Relationship; STRUCTURE-ACTIVITY RELATIONSHIPS; THERMODYNAMICS; TRACER TECHNIQUES; X-RAY DIFFRACTION
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00435a011

We have found a correlation between liposome fusion kinetics and lipid phase behavior for several inverted phase forming lipids. N-Methylated dioleoylphosphatidylethanolamine (DOPE-Me), or mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleoylphosphatidylcholine (DOPC), will form an inverted hexagonal phase (HII) at high temperatures (above TH), a lamellar phase (L alpha) at low temperatures, and an isotropic/inverted cubic phase at intermediate temperatures, which is defined by the appearance of narrow isotropic 31P NMR resonances. The phase behavior has been verified by using high-sensitivity DSC, 31P NMR, freeze-fracture electron microscopy, and X-ray diffraction. The temperature range over which the narrow isotropic resonances occur is defined as delta TI, and the range ends at TH. Extruded liposomes (approximately 0.2 microns in diameter) composed of these lipids show fusion and leakage kinetics which are strongly correlated with the temperatures of these phase transitions. At temperatures below delta TI, where the lipid phase is L alpha, there is little or no fusion, i.e., mixing of aqueous contents, or leakage. However, as the temperature reaches delta TI, there is a rapid increase in both fusion and leakage rates. At temperatures above TH, the liposomes show aggregation-dependent lysis, as the rapid formation of HII phase precursors disrupts the membranes. We show that the correspondence between the fusion and leakage kinetics and the observed phase behavior is easily rationalized in terms of a recent kinetic theory of L alpha/inverted phase transitions. In particular, it is likely that membrane fusion and the L alpha/inverted cubic phase transition proceed via a common set of intermembrane intermediates.