Absence of methylation of a CpG-rich region at the 5 prime end of the MIC2 gene on the active X, the inactive X, and the Y chromosome

The authors have identified and characterized a Hpa II tiny fragment (HTF) island associated with the promoter region of the pseudoautosomal gene MIC2. The MIC2 HTF island is unmethylated on both the active and inactive X chromosome and is similarly unmethylated on the Y chromosome. Unlike the major...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1988-08, Vol.85:15
Main Authors: Goodfellow, P.J., Mondello, C., Darling, S.M., Pym, B., Little, P., Goodfellow, P.N.
Format: Article
Language:English
Subjects:
550401 - Genetics- Tracer Techniques
ANIMALS
ANTIBODIES
ANTIGENS
BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
GENE REPRESSORS
GENES
HETEROCHROMOSOMES
INACTIVATION
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
METHYLATION
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACIDS
NUCLEOPROTEINS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
X CHROMOSOME
Y CHROMOSOME
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Description
Summary:The authors have identified and characterized a Hpa II tiny fragment (HTF) island associated with the promoter region of the pseudoautosomal gene MIC2. The MIC2 HTF island is unmethylated on both the active and inactive X chromosome and is similarly unmethylated on the Y chromosome. Unlike the majority of genes borne on the X chromosome, MIC2 fails to undergo X chromosome inactivation. HTF islands associated with X chromosome-liked genes that are inactivated are highly methylated on the inactive or transcriptionally silent homologue. The failure of MIC2 to undergo X chromosome inactivation correlates with the lack of methylation of HTF island at the 5{prime} end of the gene. These results provide further evidence that DNA methylation plays an important role in the phenomenon of X chromosome inactivation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.15.5605