Lysophosphatidylcholine metabolism to 1,2-diacylglycerol in lymphoblasts: involvement of a phosphatidylcholine-hydrolyzing phospholipase C

• Published in: Biochemistry (Easton) 1989-04, Vol.28 (7), p.2902-2909
• Main Authors: Nishijima, Junichi, Wright, Timothy M, Hoffman, Robert D, Liao, Fang, Symer, David E, Shin, Hyun S
• Format: Article
• Language: English
• Subjects: 550201 - Biochemistry- Tracer Techniques; ALCOHOLS; AMINES; AMMONIUM COMPOUNDS; ANIMAL CELLS; Animals; BASIC BIOLOGICAL SCIENCES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL PATHWAYS; BIOSYNTHESIS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBON 14 COMPOUNDS; CELL CULTURES; Cell Line; CHOLINE; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; Diglycerides - metabolism; DRUGS; ESTERS; Glycerides - metabolism; GLYCEROL; Hydrolysis; HYDROXY COMPOUNDS; ISOTOPES; Kinetics; LABELLED COMPOUNDS; LEUKOCYTES; LIGHT NUCLEI; LIPASE; LIPIDS; LIPOTROPIC FACTORS; LYMPHOCYTES; Lymphoma - enzymology; Lysophosphatidylcholines - metabolism; MATERIALS; METABOLISM; Mice; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; Phosphatidylcholines - metabolism; PHOSPHOLIPIDS; Phosphoproteins - biosynthesis; Phosphoproteins - isolation & purification; PHOSPHORUS 32; PHOSPHORUS ISOTOPES; Phosphorylation; QUATERNARY COMPOUNDS; RADIOISOTOPES; SOMATIC CELLS; SYNTHESIS; Thymus Neoplasms - enzymology; Type C Phospholipases - metabolism
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi00433a023

We have previously described the chemoattraction of lymphoblasts by lysophosphatidylcholine [Hoffman, R. D., et al. (1982) Proc. Natl. Acad. Sci. U.S.A. 79, 3285-3289]. In studying the mechanism of chemoattraction it was found that lysophosphatidylcholine was metabolized to 1,2-diacylglycerol by the lymphoblastic cell line 6C3HED. One route of metabolism involves the acylation of lysophosphatidylcholine to phosphatidylcholine with subsequent hydrolysis to 1,2-diacylglycerol and phosphocholine by the action of phospholipase C. The increase in cellular 1,2-diacylglycerol was established by metabolic experiments using [14C]glycerol-labeled lysophosphatidylcholine and by mass measurements of 1,2-diacylglycerol. The presence of a phosphatidylcholine-hydrolyzing phospholipase C was confirmed in 6C3HED cell homogenates. In intact cells, lysophosphatidylcholine induced a pattern of protein phosphorylation similar to those of 1,2-dioctanoylglycerol and phorbol 12-myristate 13-acetate, two known activators of protein kinase C. This pathway of lysophosphatidylcholine metabolism, which involves a phosphatidylcholine-hydrolyzing phospholipase C, may be important in the activation of protein kinase C independent of inositol phospholipid hydrolysis.