Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase

When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinit...

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Bibliographic Details
Published in:Biochemistry (Easton) 1989-04, Vol.28 (7), p.2768-2771
Main Authors: Hoover, Timothy R, Imperial, Juan, Ludden, Paul W, Shah, Vinod K
Format: Article
Language:English
Subjects:
550201 - Biochemistry- Tracer Techniques
BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
CARBOXYLIC ACID SALTS
Chromatography, DEAE-Cellulose
Chromatography, Gel
Chromatography, Ion Exchange
CITRATES
COENZYMES
ENZYMES
Ferredoxins - biosynthesis
HYDROGEN COMPOUNDS
Kinetics
Klebsiella pneumoniae - enzymology
MAGNETIC RESONANCE
Magnetic Resonance Spectroscopy
Molybdoferredoxin - biosynthesis
Molybdoferredoxin - isolation & purification
NITRO-GROUP DEHYDROGENASES
NITROGENASE
Nitrogenase - biosynthesis
Nitrogenase - isolation & purification
NUCLEAR MAGNETIC RESONANCE
OXIDOREDUCTASES
PURIFICATION
RESONANCE
SYNTHESIS
Tricarboxylic Acids - analysis
Tricarboxylic Acids - metabolism
TRITIUM COMPOUNDS
UPTAKE
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Description
Summary:When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at a 1:1 ratio with molybdenum.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00433a004